大豆异黄酮清斑软胶囊对小鼠B16细胞黑色素合成的影响

Effects of Qingban Capsules on Melanin Synthesis of Murine B16 Cells

目的测定大豆异黄酮清斑软胶囊对小鼠B16细胞黑色素合成的影响,研究其对黄褐斑的治疗作用。方法小鼠B16黑素细胞作为受试细胞,选择氢醌作为阳性对照物,用MTT比色法测定药物对细胞增殖的影响,采用酶学方法检测对酪氨酸酶活性的影响,NaOH溶解法测定黑素含量。结果不同浓度大豆异黄酮清斑软胶囊对黑素细胞增殖、酪氨酸酶活性及黑色素含量均有抑制作用,呈现剂量依赖性关系。结论大豆异黄酮清斑软胶囊能有效抑制黑素细胞增殖和降低酪氨酸酶活性,从而减少黑素合成。

Objective To investigate the effect of Qingban capsules which mainly contain soybean isoflavone on melanin synthesis of murine B16 cells,and study the therapeutical effect on chloasma.Methods Murine B16 cells were selected as trial objects,and hydroquinone was chosed as a positive control.The effects of drugs on cell proliferation,tyrosinase activity and melanin content were determined by MTT colorimetricassay,zymologic method and sodium hydroxide dissolution method respectively.Results Qingban capsules with different concentrations had inhibitory effect on B16 cell proliferation,tyrosinase activity and melanin content in a dose-dependent manner.Conclusion Qingban capsules could effectively inhibit B16 cell proliferation,reduce tyrosinase activity,so as to reduce melanin synthesis.