湿润烧伤膏中脂肪酸的气相色谱分析

Analysis of Fat Acid in Moist Expose Burn ointment by Gas chromatography

目的 :了解湿润烧伤膏的脂肪酸组成特性。方法 :气相色谱分析法。色谱仪为日本岛津GA 1 4A气相色谱仪 ,氢离子火焰检测器 (FID) ,C RA4数据处理机 ,色谱柱为 4 0mm× 2m玻璃柱 ,内填 1 5%丁二酸二乙二醇聚酯 (DEGS) /62 0 1 ,80 1 0 0目固定相。色谱条件 :柱温 1 80 2 0 0℃ ;进样口温度 2 50℃ ;检测器温度 2 50℃ ;氮气流速 40ml/min ;氢气流速 35ml/min ;空气流速 30 0ml/min。进样量 0 .5μl。结果 :发现湿润烧伤膏的脂肪酸组成中 ,油酸和亚油酸之和约占 80 %以上。结论 :湿润烧伤膏较好的临床疗效和其脂肪酸组成中亚麻酸的高比例含量之间可能有关 ,值得进一步研究。

Objective:To analysis and find out the component of fat acids in Moist Expose Burn Ointment (MEBO). Method:Gas chromatography. SHIMADZU Model GA 14A gas chromatography. It is equipped with a flame ionization detector (FID), and C RA 4 integrator. The column were 4.0mm×2.0m glass capillary columns coated with 15% DEGS/6201, 80 100 mesh solid phase. The chromatographic conditions were: injection port temperature 250℃; column temperature 180 200℃; detector temperature 250℃;nitrogen velocity of flow at 40ml/min, hydrogen at 35 ml/min, air at 300 ml/min; sample size 0.5μl. Result:The quantity of oleic acid and linoleic acid contained in MEBO is more than 80% in total fat acid. Conclusion:The relation between the excellent clinic therapeutical effects of MEBO and the high quantity of linoleic acid in MEBO is needed further studies. [