表型筛选试验检测阴沟肠杆菌产β-内酰胺酶及耐药分析

Improved Phenotype Screening Test for the Detection of Enterobacter Cloacae Producing β-lactamase and Drug Resistance Analysis

目的:对近年耐三代头孢的阴沟肠杆菌进行β-内酰酶(主要是ESBLs与AmpC酶)监测以进行流行病学调查,同时作相关统计以指导实验室检测及临床用药。方法:收集来自各种标本对三代头孢耐药的阴沟肠杆菌45例表型筛选试验检测ESBLs与AmpC酶。结果:产ESBLs 42株,占93.33%,产AmpC酶8株,占17.78%,两种酶均产的有8株。结论:对三代头孢耐药的阴沟肠杆菌主要产ESBLs,体外实验对亚胺培南敏感率为97.66%,对其他抗菌药物敏感性差。

Objective： To serveil β-lactama（mainly the extended Enterobacter cloacae resistant to the third genus cephalosporins spectrum β-lactarna and AmpC enzyme） produced by in order to process epidemiological investigation, and to provide laboratory and physicians with the statistical analysis of antibiotics susceptibility. Methods： We collected 45 strains of Enterobacter cloacae resistant to the third genus cephalosporins from all kinds of specimens, then detect ES- BLs and AmpC enzyme with phenotype screening test. Results：Among 45 strains of enterobacter cloacae, there were 42 strains（93. 33%） producing ESBLs in three-dimensional test. But there were 8 strains（17. 78%） producing the AmpC enzyme in the test. There were 8 strains producing the two types of enzymes in test. Conclusion： Enterobacter cloacae resistant to the the third genus cephalosporins produces ESBLs really. There is a susceptibility rate of 97. 66% to imi- penem for it in vitro,but it is less susceptive to other antibiotics.