麦门冬汤对放射性肺损伤大鼠肺组织TGF-β、TNF-α表达的影响

Effect of Maimendong Decoction on TGF-β、TNF-αExpression of Lung Tissues In A Rat Model of Adiation-Induced Lung Injury

目的探讨麦门冬汤对放射性肺损伤大鼠肺组织TGF-β、TNF-α表达的影响。方法将72只健康成年清洁级雌性Wistar大鼠随机分为空白对照组(A组)24只,单纯照射组(B组)24只,照射加中药组(C组)24只,A、B两组以蒸馏水2ml/200g体重灌胃一周后,C组以每日2ml/200g体重(1.6g/200g体重)灌胃服用中药一周,开始照射,照射期间,每周测体重一次,依照上述剂量,A、B组以蒸馏水、C组以中药继续灌胃至照射结束。分别在完成10次照射后(第5周),以及在照射开始后的第12、26周,检测各组大鼠肺组织TGF-β、TNF-α表达。结果①TGF-β蛋白表达:B组大鼠TGF-β于照射第5周时出现表达达到高峰;在各时问点,A、C组大鼠TGF-β表达均低于B组(P<0.01);②TNF-α表达:B组大鼠肺组织TNF-α的表达在第5周时达到高峰,C组在第5周时明显抑制了TNF-α表达,在第5周、12周、26周,C组大鼠TNF-α表达均明显低于B组(P<0.01)。结论中药麦门冬汤具有较好的预防放射性肺损伤功效,其作用机制与阻抑肺组织TGF-β、TNF-α表达有关。

Objection To explore 1 effect of maimendong decoction on TGF-β,TNF-αexpression of lung tissues. Methods 84 Wistar rats were randomly divided into the 3 groups （24 each） （group A normal control group, group B model control group, group C the Chinese medicine, 5,12,26 week after irradiation in right lung, then observed and investigated the histological change of lungs index and expression of TGF-β,TNF-α Expression of Lung Tissues. Results TGF-βand TNF-α were expressed obviously in the irradiation group since the fifth week, Chinese medicine groups can effectively prevent the expression of TGF-β and TNF-α. Conclusion Maimendong Decoction shown the effect of Preventing and treating addition-Induced Lung Injury and its mechanism is related to repress the expression of TGF-β, TNF-α expression of lung tissues.