拮抗放线菌F1发酵液抑菌谱及稳定性分析

Analysis to the inhibitory spectrum and stability of fermentation broths of actinomycete strain F1

对拮抗放线菌菌株F1发酵液的抑菌谱及稳定性做了研究。抑菌活性实验表明:菌株F1发酵液对供试17种植物病原真菌和7种病原细菌都有较强的抑制活性,在供试的植物病原真菌中,对番茄灰霉病菌和李干腐病菌的抑制作用最强,抑菌圈直径分别为41.04mm和40.12mm;病原细菌中,对丁香假单胞菌流泪致病变种的抑制作用最强,抑菌圈直径可达22.15mm;稳定性实验结果表明:菌株F1发酵液对紫外线、热和酸碱较稳定,在pH为6～10条件下,均能保持较强的抑菌活性,但不耐强酸、强碱;当温度低于60℃时,处理30min和60min,抑菌活性几乎没变化,仍能保持较强的抑菌效果,但高于80℃时,抑菌活性明显降低。表明该菌株发酵液具有广谱抑菌活性和较强的稳定性,具有一定开发价值。

The inhibitory spectrum and stability of fermentation broths of actinomycete strain F1 were studied. The result of antimicrobial activity experiment showed： the fermentation broths had significant inhibitory effect on 17 tested species of plant pathogenic fungi and 7 tested species ot bacteria. The fermentation broths showed the strongest antimicrobial effect against Botrytiscinerea and Polvporus hirsutus among 17 fungi species. The inhibitory diameters of Botrytiscinerea or Polvporus hir-sutus were 41.04mm and 40.12 mm, respectively. The antimicrohial diameter of Pseudomonas syringae pv. lachrymans was the biggest （22.15mm） among 7 tested bacteria species. The stability of fermentation broths was stable to UV, acid, alkali and heat. The fermentation broths remained high antimicrobial effect at pH6 - 10, but unstable to strong acid and alkali. There was no obvious change on the antimicrobial effect of fermentation broths from 20℃ to 60℃ for 30min and 60min, but the inhib- itory activity of fermentation broths decreased considerably at 80℃ or above.