摘要
对拮抗放线菌菌株F1发酵液的抑菌谱及稳定性做了研究。抑菌活性实验表明:菌株F1发酵液对供试17种植物病原真菌和7种病原细菌都有较强的抑制活性,在供试的植物病原真菌中,对番茄灰霉病菌和李干腐病菌的抑制作用最强,抑菌圈直径分别为41.04mm和40.12mm;病原细菌中,对丁香假单胞菌流泪致病变种的抑制作用最强,抑菌圈直径可达22.15mm;稳定性实验结果表明:菌株F1发酵液对紫外线、热和酸碱较稳定,在pH为6~10条件下,均能保持较强的抑菌活性,但不耐强酸、强碱;当温度低于60℃时,处理30min和60min,抑菌活性几乎没变化,仍能保持较强的抑菌效果,但高于80℃时,抑菌活性明显降低。表明该菌株发酵液具有广谱抑菌活性和较强的稳定性,具有一定开发价值。
The inhibitory spectrum and stability of fermentation broths of actinomycete strain F1 were studied. The result of antimicrobial activity experiment showed: the fermentation broths had significant inhibitory effect on 17 tested species of plant pathogenic fungi and 7 tested species ot bacteria. The fermentation broths showed the strongest antimicrobial effect against Botrytiscinerea and Polvporus hirsutus among 17 fungi species. The inhibitory diameters of Botrytiscinerea or Polvporus hir-sutus were 41.04mm and 40.12 mm, respectively. The antimicrohial diameter of Pseudomonas syringae pv. lachrymans was the biggest (22.15mm) among 7 tested bacteria species. The stability of fermentation broths was stable to UV, acid, alkali and heat. The fermentation broths remained high antimicrobial effect at pH6 - 10, but unstable to strong acid and alkali. There was no obvious change on the antimicrobial effect of fermentation broths from 20℃ to 60℃ for 30min and 60min, but the inhib- itory activity of fermentation broths decreased considerably at 80℃ or above.
出处
《北京农学院学报》
2012年第1期32-35,共4页
Journal of Beijing University of Agriculture
基金
北京市教委资助项目(KM200910020001)
北京市自然科学基金资助项目(5112010)
关键词
放线菌
发酵液
抑菌谱
稳定性
actinomyeete
fermentation broths
inhibitory spectrum
stability