摘要
目的 探讨水通道1蛋白（AQP1）在胃癌组织中的表达及其与细胞凋亡、微血管密度的关系和临床意义.方法 取89例胃癌组织标本（胃癌组）及同期21例胃正常组织标本（对照组）,检测比较两组AQP1的表达、细胞凋亡情况及微血管密度.同时分析AQP1与胃癌预后的关系.结果 对照组AQP1阳性表达者仅2例（9.5%）,胃癌组AQP1阳性表达者61例（68.5%）,差异有统计学意义（P＜0.01）.胃癌组中伴淋巴结转移者AQP1蛋白表达水平明显高于无淋巴结转移者（P＜0.05）.AQP1表达强度与微血管密度显著正相关（P＜0.05）;AQP1阳性表达者细胞凋亡率明显低于AQP1阴性表达者（P＜0.05）.AQP1阳性表达者术后生存期明显短于AQP1阴性表达者（P＜0.05）.Cox多元回归分析显示,AQP1表达、有无淋巴结转移是预后的独立影响因素（均P＜0.05）.结论 AQP1的高表达与胃癌恶性进展有关,其机制可能是通过抑制细胞凋亡和肿瘤血管形成;AQP1可以作为判断胃癌预后的临床指标.
Objective To investigate the expression of AQP1 in gastric cancer and its relation to cell apoptosis, angiogenesis and prognosis of patients. Methods The expression of AQP1 was examined in 89 tissue samples of gastric cancer and 21 samples of normal gastric mucosa by immunohistochemical method. The tissue microvessel was immunostained with anti-human CD34 antibody; both the expression of AQP1 and microvessel density （MVD） were counted by the image analysis. The apoptosis rate was tested by flow cytometry. Results The positive AQP1 expression rate was 9.5%（2/21） in normal gastric mucosa and 68,5%（61/89） in gastric cancer（P〈0.01）. The expression of AQP1 in gastric cancer was significantly correlated with lymphatic metastasis,tissue MVD and patients survival（P〈0.05）. Multivariate analysis revealed that AQP1 expression and lymph node metastasis were independent prognostic factors of poor survival（P〈0.05）. Conclusion AQP1 is an independent prognostic factor of gastric carcinoma, which may be associated with the inhibition of cell apoptosis and tissue angiogenesis in gastric cancer.
出处
《浙江医学》
CAS
2012年第6期405-407,共3页
Zhejiang Medical Journal
基金
温州市科技计划项目（Y20110023）
