摘要
目的 探讨高压氧联合复方海蛇胶囊对脑梗死大鼠的空间学习记忆、皮质和海马神经胶质原纤维酸性蛋白（GFAP）mRNA表达的影响.方法 将50只SD大鼠分为5组,用线栓法建立大鼠大脑中动脉栓塞模型.以高压氧和（或）复方海蛇胶囊处理4周后行Morris水迷宫实验,观察大鼠寻找站台的平均逃避潜伏期和游泳总距离及平均游泳速度;RT-PCR法测定大鼠海马及皮质区GFAP mRNA的表达水平.结果 （1）Morris水迷宫实验显示,高压氧和（或）复方海蛇胶囊干预可使大鼠寻找站台的平均逃避潜伏期和游泳总距离减少（P＜0.05）;（2）RT-PCR检测显示,海马及皮层区GFAPmRNA表达水平在高压氧和（或）复方海蛇胶囊干预后显著降低（P＜0.05）.结论 高压氧和（或）复方海蛇胶囊可改善脑梗死大鼠的空间学习记忆能力,并减少海马和皮质区GFAPmRNA的表达.
Objective To investigate the effects of hyperbaric oxygen therapy combined with administration of Fufang Haishe capsules on learning-memory and the expression of glial fibrillary acid protein（GFAP） mRNA in rat model of cerebral infarction. Methods Cerebral infarction was induced by middle cerebral artery occlusion（MCAO） in 50 rats, the rate were exposed to hyperbaric oxygen and/or Fufang Haishe capsules therapy at the end of 4th week. The mean escape latency and swim path distance and the mean swim speed of rats were assessed with Morris water maze; the expressions of GFAPmRNA in the hippocampus and cortex of rats were determined by RT-PCR. Results In the Morris water maze task, the mean escape latency and swim path length of rats with hyperbaric oxygen and/or Fufang Haishe capsules therapy were shorter than those of control group （P〈0.05）. The expression of GFAPmRNA was decreased in rats with hyperbaric oxygen and/or Fufang Haishe capsules therapy than that of control group （P〈0.05）. Conclusion Hyperbaric oxygen and/or Fufang Haishe capsules therapy can improve spatial learning-memory ability and decrease the mRNA expression of GFAP in the hippocampus and the cortex of rats with cerebral infarction.
出处
《浙江医学》
CAS
2012年第6期427-430,共4页
Zhejiang Medical Journal
基金
浙江省中医药管理局课题资助项目（Y200813486）
