丙型肝炎病毒非结构区抗原制备及其相应抗体的检测

Preparation of hepatitis C virus nonstructural region antigens and detection of their relative antibodies

目的 研究丙型肝炎病毒非结构区 3(HepatitisCvirusnonstructurdregion 3,HCV NS3)与丙型肝炎发病的关系 ,寻求一种可用于判断临床干扰素 (Interferon ,IFN)治疗疗效的检测方法。方法 采用基因重组 ,经大肠埃希菌表达 ,提取HCV NS3不同末端蛋白作为抗原 ,并回顾性的对IFN治疗的 6 3例HCV RNA阳性的丙型肝炎患者血清用免疫印迹 (Westernblot)方法 ,进行了相应抗体检测。结果 证实HCV NS3不同末端抗体在丙型肝炎患者 ,特别是慢性丙型肝炎患者 ,与IFN治疗疗效关系密切。结论 通过测定HCV NS3不同末端抗体的方法有可能对丙型肝炎 ,特别是慢性丙型肝炎患者的IFN治疗效果进行评价 ,为预测疗效提供了一个新的手段和依据。

Objective To investigate the relationship between HCV NS3 and the pathogenesis of HCV and research a method to assess the therapeutic effect of interferon (IEN) in clinicl.Methods In a retrospective research,we used HCV NS3 antigens of different terminals expressed in E. coli. in which contained the recombinant plasmids involving amino terminal region (N′) or carboxyl terminal region(C′) of HCV NS3, to detect the antibodies by Western blot in sera of 63 hepatitis C patients who were treated with IFN.Results It proved that antibodies against HCV NS3 N′ and/or HCV NS3 C′ terminals in hepatitis C patients may predict the treatment result of IFN, especially in the patients with chronic hepatitis C.Conclusion The detection of antibodies against HCV NS3 N′ and/or C′ terminals may be used for evaluation of IFN treatment, especially in chronic hepatitis C ones.