端粒酶在瘢痕发生发展过程中的表达及意义

Expression and significance of telomerase in development process of scar formation

目的通过研究瘢痕发生、发展过程中成纤维细胞中端粒酶的表达，探讨端粒酶与瘢痕发生、发展的关系。方法采用免疫组织化学染色法（PV-6000二步法）。实验分为5组：肉芽组织组18例、瘢痕疙瘩组17例、增生性瘢痕组16例、成熟性瘢痕组28例、正常皮肤组32例，对标本成纤维细胞端粒酶活性进行检测，用SPSS16．0进行统计学分析。结果肉芽组织组成纤维细胞端粒酶阳性表达率为9d．4％，瘢痕疙瘩组阳性丧达率为58．8％，增生性瘢痕组阳性率为18．8％，成熟性瘢痕组阳性率为0。正常皮肤组阳性率为0，各纰间两阿比较，除成热性瘢痕组和正常皮肤组外。其余各组差别均有统计学意义（P〈0．05）。结论瘢痕的形成是一个多闪素参与综合作用的结求．而端粒酶的激活是其中一个重要的因素。通过榆测搬痕发生、发展过科中的端粒胁活性，有可能削断搬痕的预后；通过涮拄瘢痕发生、发展过程中端粒酶的活性，有可能成为壤痕治疗的新途径。

Objective To study the expression of telomerase of fihroblasls in the formation and development of scar, and to investgate the relationship between the telomerase activity and the formation and development of scar. Methods Expression of telornerase was detected by immunohistochemical techniques in 18 specimens from granulation lissue, 17 keloid, 16 specimens from hypertrophic scar, 28 specimens from mature scar, 32 specimens from normal skin, and SPSS16.0 statistics software was used toanalyze the relationship between telomeraseand scar. Results It was shown that the positive expression rate of telomerase in the granulation tissue group was 94.4 %, that in the keloid group was 58.8 %, that in the hypertrophic scar group was 18.8 %, and 1hat in the mature scar and in the normal skin was zero. comparisons between groups, in addition to the normal scar group find normal skin group, other groups were statistically significant differences. Conclusions Scar formation is a process with muhi-factor participation, while telomerase activation is an important factor. Derecting telomerase activity in the development process of scar formation may determine the prognosis. Through the control of telomerase activity in the pathogenesis of scar may become a new approach of scar treatment.