参麦注射液与氨茶碱合用对家兔膈肌功能的影响

Effects of the Combination of Shenmai Injection and Aminophylline on Diaphragmatic Function in Rabbits

目的：观察参麦注射液与氨茶碱联合用药对兔跨膈压及膈肌电活动影响．提高隔肌疲劳的治疗效果。方法：双侧膈神经刺激４０ｍｉｎ，复制膈肌疲劳模型（Ｄｉａｐｈｒａｇｍａｔｉｃｆａｔｉｇｕｅ，ＤｉＦ）。动物随机分３组：①参麦组（２ｍＬ／ｋｇ，Ｎ＝７）；②氨茶碱组（２０ｍｇ／ｋｇ，Ｎ＝７）；③合用组（参麦注射液２ｍＬ／ｋｇ＋氨茶碱２０ｍｇ／ｋｇ，Ｎ＝７）。于药前及药后 ３０、６０、１２０ ｍｉｎ记录跨膈压（Ｔｒａｎｓｄｉａｐｈｒａｇｍａｔｉｃ ｐｒｅｓｓｕｒｅ，Ｐｄｉ）；记录膈肌肌电图（Ｄｉａｐｈｒａｇｍａｔｉｃ ｅｌｅｃｔｒｏｍｙｏｇｒｍ，ＥＭＧｄｉ）输入计算机作快速富利叶分析（ＦＦＴ），算出高／低频比值（Ｈｉｇｈ／Ｌｏｗ ｆｒｅｑｕｅｎｃｙ ｒａｔｉｏ，Ｈ／Ｌ）、中心频率（ＣｅｎｔｒａＬ ｆｒｅｑｕｅｎｃｙ，Ｆｃ）；同时记录膈肌诱发电位（Ｄｉａｐｈｒａｇｍ ｅ－ｖｏｋｅｄ ｐｏｔｅｎｔｉａｌ，ＤＥＰ），打印出幅度和潜伏期，数据经方差分析、ｑ检验。结果：参麦注射液、氨茶碱单用均可提高Ｐｄｉ、Ｈ／Ｌ、Ｆｃ及ＤＥＰ幅度（与ＤｉＦ比，Ｐ＜０．０５）；两药合用，能提高 Ｐｄｉ、Ｆｃ，且缩短 ＤＥＰ潜伏期（合用与单用比，Ｐ＜０．０５）?

Objective: Effects of Shenmai injection and aminophylline alone or in combination on transdiaphragmatic pressure and electrical activity of diaphragm were observed, so as to evaluate their effecfs on diaphragmatic fatigue. Methods: The diaphramatic fatique (DiF) model was estab- lished by stimulating bilateral phrenic nerves for 40 minutes. The animals were allocated to three groups: Shenmai group (2mL/kg, N=7), aminophylline group (20mg/kg, N=7) and combi- nation group (Shenmai injection 2mL/kg+aminophylline 20mg/kg, N=7) The transdi- aphragmatic pressure (Pdi) and diaphragmatic electromyogram (EMGdi) were recorded before and after treatment. EMGdi was analysed by computer and then the high/low frequency ratio (H/L) and the central frequency (Fc) were caculated. The diaphragm evoked potential (DEP) was record- ed simutaneously. All data was analysed by analysis of variance and q test. Results: Pdi, H/L, Fc and amplitude of DEP were markedly increased after the treatment with aminophyline and Shen- mai injection alone (Compared with DiF. P<0.05), Pdi and Fc were increased and latent period of DEP was decreased in combination group as compared with Shenmai group and aminophylline group (P<0.05). Conclusion The aminophylline and Shenmai Injection can treat diaphragmatic fatique. The effect of their combination is better than that by using alone.