摘要
采用免疫印迹（Ｗｅｓｔｅｒｎ－ｂｌｏｔ）技术，对Ｐａｎａｘ属西洋参、人参及三七进行聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）及Ｗｅｓｔｅｒｎ－ｂｌｏｔ指纹图谱分析。确定了 Ｐａｎａｘ属药材的特征指纹图谱。提示 Ｗｅｓｔｅｒｎ－ｂｌｏｔ可用于Ｐａｎａｘ属药材，特别是西洋参的专属性鉴别。
Proteins from dried roots of three medicinal Panax species (P. Quinquefolius, P. ginseng and P. nologinseng) were analyzed by SDS-PAGE and Western-blot. The protein fin- gerprints of Panax species were confirmed. From 28KD to 58KD and 55KD are in common. Very specific fingerprints of P. quinquefolius are found below 28KD. The fingerprints above 58KD seemed to be peculiar to P. ginsing. Hence the Western-blot approach can be used as a means to authenticate the concerned Panax species, especially for identification of P. quinquefolius.
出处
《中药新药与临床药理》
CAS
CSCD
2000年第3期164-166,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省自然科学基金资助项目(950761)
关键词
免疫印迹技术
西洋参
人参
三七
中药鉴别
@Western-blot/application
P. Quinquefolius/chemistry P. ginseng/chemistry
P. nologinseng/chemistry @ Pharmacognostical identification
