人血白蛋白包裹猪肺动脉脱细胞纤维组织方法的建立

Studies on approaches of human albumin covering decellularized fiber tissue

目的:建立以人血白蛋白包裹脱细胞猪肺动脉纤维支架的方法。方法:采用自主研制的"脱水浸泡法"将人血白蛋白渗透至脱细胞猪肺动脉纤维支架内,比较脱细胞纤维组织和经白蛋白包裹的脱细胞纤维组织在物理性能方面的异同。通过免疫组织化学染色法评价白蛋白包裹脱细胞组织的效果。结果:采用"脱水浸泡法"能够使白蛋白有效地结合在脱细胞纤维组织内,完成人血白蛋白对脱细胞纤维组织的全面包裹。结论:采用"脱水浸泡法",人血白蛋白能够充分包裹脱细胞纤维支架,同时人血白蛋白包裹的脱细胞纤维组织的物理性能优于单纯脱细胞纤维组织。

AIM： To observe the approach of human albumin coveting decellularized fiber tissue. METHODS： Human albumin was penetrated into decellularized porcine pulmonary fiber scaffolds by the method of ＂ dewatering and soaking. ＂ We next examined the similarities and differences of physical features between decellularized tissue and human albumin-decellularized tissue. The effects of human albumin covering decellularized tissue were evaluated by immunohistochemical methods. RESULTS： Using the ＂dewatering and soaking＂ method, human albumin could effectively combine with decellu- larized porcine pulmonary fiber scaffolds. Human albumin completely covered decellularized tissue. CONCLUSION： Using the ＂dewatering and soaking＂ method, human albumin may effectively combine with decellularized porcine pulmonary fiber scaffolds. Human albumin-decellularized tissue was superior in physical features to decellularized tissue.