大孔树脂法纯化柑橘皮中的橙皮甙及其油脂抗氧化活性研究

Study on the Purification of Hesperidin from Orange Peels by Macroporous Resin and its Antioxidative Activity on Oil

为研究柑橘皮橙皮甙的纯化工艺及其抗油脂氧化活性,以吸附率和解吸率为考察指标,分别用AB-8、NKA-9、D-100、HP-20、HPD-400、HPD-500、HPD-6007种型号的大孔树脂对柑橘皮橙皮甙进行分离纯化,并通过碘量法检测橙皮甙抗油脂氧化活性。结果表明,AB-8型大孔树脂对柑橘皮橙皮甙有良好的吸附,其最佳吸附分离工艺条件为:药液质量浓度0.6mg/mL,上样量为3倍柱体积,上样液pH值3.0,以1.0mL/min吸附速率进行吸附,3倍柱体积的60%乙醇洗脱。橙皮甙对动物油和植物油显示不同的抗氧化活性,对猪油的抗氧化作用具有剂量效应关系。而维生素C对橙皮甙具有明显的协同抗氧化作用。作为天然的油脂抗氧化剂,橙皮甙在食品工业上具有良好的应用价值。

In this paper, seven types of macroporous resin, including AB-8, NKA-9, D-100, HP-20, HPD-400, HPD-500 and HPD-600, were used to purify the hesperidin from orange peels. Antioxidation activity of hesperidin from orange peels was studied by the iodine method. The results showed the absorption capacity and elution ratio of AB-8 resin were the best among these types of resins in static tests. The optimum absorbing and separating conditions were as follows： sample solution concentration was 0.6mg/mL; the pH value was 3.0; processing volume was 3BV; flow rate was 1.0 mL/min; elntion solvent ethanol-water（60%） solution was 3BV. Hesperidin showed different antioxidant effects in ani- mal oils and vegetable oils. In addition, ascorbic acid had synergistic antioxidant effect with hesperidin in lard. As a natural oil antioxidant supplement, hesperidin has applicational value in food industry.