摘要
目的对中药大黄的有效成分进行快速分析。方法选用Waters XBridge C18柱为分析柱,流动相为乙腈与甲酸水梯度洗脱系统,流量为300μL/min;质谱条件选用电喷雾离子源(ESI),检测方式为负离子多级质谱全扫描,子离子采用动态排除法(DDS)。结果对总离子流图的主要色谱峰的多级碎片进行解析,结合对照品的裂解规律,综合紫外(UV)数据、保留时间及参考文献,共鉴别出了中药大黄中的14个化学成分。结论采用本方法可用于中药大黄的化学成分的快速分析与鉴定,HPLC-MS联用技术值得在中药药物分析与质量控制研究中应用和推广。
Objective To analyze the active constituents of rheum palmatum L. rapidly. Methods The samples were separated on a Waters XBridge C18 column. The mobile phase consisted of acetonitrile and water （formic acid） using gradient elution and flow rate was set at 300 μL/min. ESI source was hired and negative mode was chosen in MS/MS detection. Results Fourteen phenolic compounds were identified based onmass spectra, UVdata and comparison with literatures. Conclusions The rapid HPLC-DAD-MS/MSmethod is established for analysis of Rheum Palmatum L. and it indicates the relevance and usefulness of the combination of HPLC and MS to identify constituents in TCM.
出处
《临床医学工程》
2012年第4期530-531,共2页
Clinical Medicine & Engineering
