结直肠癌向肝微转移的观察

Detection of Micrometastasis of Liver in Colorectal Cancer Patients

目的 :探讨结直肠癌患者在手术时癌细胞向肝发生微转移的情况。方法 :以癌胚抗原(CEA)表达及角化蛋白19(K19)表达癌细胞为标记物 ,应用巢式反转录聚合酶链反应 (NestedRt-pcr)对55例术中患者 ,并以术后患者及正常人各12例为对照进行了检测。结果 :两项标记物皆阳性者在临床 (Duke's)早期已经出现 ,随分期之进展而逐步增高。并随癌细胞恶性程度升高而上升。结论 :用门静脉血检测结直肠癌患者在手术时癌细胞向肝发生微转移的情况比其它方法阳性率更高 。

Objective: To study the micrometastasis of liver in colorectal cancer operation. Methode: Using carcinoembryonic antigen (CEA) expression and kerating 19(K19) expression tumor cells as marker to detect micrometastasis of liver in 55 patients in operation, 12 postoperative patients and 12 normal persons as control by nested reverse transcriptase polymerase chain reaction (RT-PCR). Results: The liver micrometastasis in patients with double marker-positive appeared in the early Duke′s stage and it escalated along with the rising of the Duke′s stage and progression of malignant change. Conclusion: To detect micrometastasis of liver in portal vein blood during operation of colorectal cancer is more favorable than other methods.