回直肠吻合分流术对慢传输型便秘大鼠血浆SP及VIP的影响

Effect of colonic bypass with ileorectal anastomosis on plasma levels of SP and VIP in rats with slow transit constipation

目的:完成STC大鼠回肠直肠吻合分流手术,观察该术式对STC大鼠血浆SP、VIP的影响.方法:72只SD大鼠,随机取10只作为正常对照组,其余62只用大黄小剂量递增灌胃造模.造模过程中死亡5只,剩余57只,手术前处死12只作为模型对照组.剩余的45只大鼠,随机35只手术组,10只自然恢复组,测定并比较各组大鼠血浆中SP及VIP的含量.结果:SP水平:与正常对照组相比,模型组大鼠血浆SP水平显著降低(63.364±4.211vs81.032±4.237,P<0.01);恢复组对比模型组SP水平降低显著(50.138±5.283vs63.364±4.211,P<0.01);术后1mo,手术组对比恢复组数值增高(58.165±6.592vs50.138±5.283,P<0.05);但仍然低于模型组(58.165±6.592vs63.364±4.211,P<0.05).VIP水平:与正常对照组相比,模型组大鼠血浆VIP水平显著升高(32.152±6.204vs25.469±4.523,P<0.01);恢复组较模型组下降(25.217±3.517vs32.152±6.204,P<0.05),且与正常对照组无显著差异.手术组对比恢复组无显著差异.结论:回直肠吻合分流术明显改善STC大鼠的便秘症状,减轻结肠负担后能减轻结肠功能的进一步恶化,但能否促进大鼠结肠功能恢复尚待进一步研究.

AIM： To investigate the influence of colonic bypass with ileorectal anastomosis on plasma levels of substance P （SP） and vasoactive intestinal peptide （VIP） in rats with slow transit constipation （STC）. METHODS： A total of 72 rats were used in the study, of which 10 were included in normal control group, and 62 were used to induce STC by intragastric administration of gradually increasing doses of rhubarb suspension. STC was successfully induced in 57 rats, and 12 of them were used as model controls and killed before operation. The remaining 45 rats were randomized into operation group （n = 35） and recovery group （n = 10）. Plasma levels of SP and VIP were measured in each group. RESULTS： SP： Compared to the normal control group, plasma levels of SP decreased significantly in the model group （63.364 ± 4.211 vs 81.032 ± 4.237, P 0.01）. Plasma levels of SP were lower in the recovery group than in the model group （50.138 ± 5.283 vs 63.364 ± 4.211, P 0.01）, but were higher in the operation group than in the recovery group （58.165 ± 6.592 vs 50.138 ± 5.283, P 0.05）. Compared to the normal control group, plasma levels of VIP increased significantly in the model group （32.152 ± 6.204 vs 25.469 ± 4.523, P 0.01）. Plasma levels of VIP were lower in the recovery group than in the model group （25.217 ± 3.517 vs 32.152 ± 6.204, P 0.05）, but showed no significant difference between the normal control group and recovery group. CONCLUSION： Colonic bypass with ileorectal anastomosis significantly improves symptoms and reduces the further deterioration of colonic function in STC rats.