摘要
目的:探讨肝细胞癌(HCC)患者外周血中甲胎蛋白(AFP)、端粒酶逆转录酶(h-TERT)及血管内皮生长因子(VEGF) mRNA的表达及联合检测对诊断HCC的应用价值.方法:应用荧光定量PCR(FQ-PCR)对40例HCC、20例肝外良性疾病及10例正常体检人群的外周血AFP mRNA、h-TERT mRNA及VEGF mRNA的表达量进行检测.结果:3种指标在HCC患者中阳性率分别为77.5%、85%及72.5%,与对照组相比差异有统计学意义(P<0.01).且AFP mRNA、VEGF mRNA的阳性表达与HCC患者血清AFP水平无关(P>0.05),h-TERT mRNA与患者血清AFP水平存有相关性(P=0.01);3种指标与HCC患者的TNM分期高度相关(P<0.01).AFP mRNA在诊断HCC特异性较高,而AFP mRNA和h-TERT mRNA联合检测效果更好(敏感度92.%,特异度80%).结论:3种指标对诊断HCC均能起到良好的参考作用,联合检测AFP mRNA及h-TERT mRNA能增加诊断HCC的敏感度及特异度.
AIM: To evaluate the diagnostic significance of alpha-fetoprotein (AFP), human telomerase reverse transcriptase (h-TERT) and vascular endothelial growth factor (VEGF) mRNAs in peripheral blood of patients with hepatocellular carcinoma (HCC). METHODS: Fluorescence quantitative PCR (FQ-PCR) was used to detect the levels of AFP, h-TERT and VEGF mRNAs in peripheral blood samples from 40 patient with HCC, 20 patients with liver disease and 10 normal controls. RESULTS: The positive rates of all three parameters were significantly higher in HCC patients than in controls (all P 0.01). The positive expression of AFP and VEGF was not correlated with serum AFP levels in HCC patients (P 0.05), whereas h-TERT mRNA had a correlation with serum AFP levels (P = 0.01). The positive rates of all three parameters were highly correlated with TNM stage in HCC patients (all P 0.01). The specificity of AFP mRNA in the diagnosis of HCC was higher, and combined detection of AFP and h-TERT mRNAs had a higher accuracy. CONCLUSION: AFP, h-TERT and VEGF mRNAs in peripheral blood can be used as auxiliary parameters for the diagnosis of HCC, and combined detection of AFP and h-TERT mRNAs in peripheral blood can increase the sensitivity and specificity for diagnosing HCC.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第7期610-614,共5页
World Chinese Journal of Digestology
基金
首都医学发展科研基金资助项目
No.2007-3024~~
关键词
肝细胞肝癌
荧光定量聚合酶链式反应
甲胎蛋白
端粒酶逆转录酶
血管内皮生长因子
Hepatocellular carcinoma
Fluorescence quantitative polymerase chain reaction
Alpha-fetal protein
Human telomerase reverse transcriptase
Vascular endothelial growth factor