MTHFR基因多态性与胃癌的相关性

Association between methylenetetrahydrofolate reductase gene polymorphisms (C677T and G1793A) and risk of gastric cancer

目的:探讨MTHFR基因多态性与胃癌发病风险的相关性.方法:收集进展期胃癌患者97例,应用PCR对C677T、G1793A多态位点进行检测;采用病例对照研究设计,比较病例组和对照组多态位点基因频率和基因型频率.结果:677T等位基因频率在病例组和对照组中差异无显著性意义,病例组CC基因型频率、CT基因型频率、TT基因型频率与对照组相应基因型频率差异无显著性意义;1793A等位基因频率在病例组和对照组中差异无显著性意义,病例组GG基因型频率、GA基因型频率与对照组相应基因型频率差异无显著性意义.结论:MTHFR多态性可能参与了胃癌的发生、发展,但他仅是导致胃癌发生的因素之一.因此研究MTHFR多态性与胃癌发生之间的关系,有必要对胃癌病因进行分类,MTHFR多态性可能参与了某一类胃癌的发生.

AIM： To explore the association between two non-synonymous single nucleotide polymorphisms （C677T and G1793A） of the methylenetetrahydrofolate reductase （MTHFR） gene and risk of gastric cancer. METHODS： Ninety patients with advanced gastric cancer and 114 normal controls were included in the study. DNA samples isolated from peripheral blood which were used to genotype C677T and G1793A polymorphisms by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. RESULTS： No significant differences in allele frequencies and genotype frequencies were found between the patients and controls, suggesting that there might be no association between the two MTHFR gene polymorphisms and risk of gastric cancer. CONCLUSION： It is necessary to classify gastric cancer according to causes of disease to investigate the association between MTHFR gene polymorphisms and risk of this disease since MTHFR gene polymorphisms may be associated with only some subtypes.