钼对人食管癌细胞ECA-109的化疗增敏作用及对食管癌干细胞p75^(NTR)的影响

Molybdenum increases chemosensitivity of ECA-109 cells to cisplatin and cisplatininduced inhibition of p75^(NTR) expression in esophageal stem cells

目的:探讨钼对食管癌细胞ECA-109化疗敏感性的影响及对食管癌干细胞p75NTR作用.方法:本实验选用人食管癌细胞(esophageal cancer cells,ECCs)ECA-109.设计分为4组:空白对照组、顺铂组、单纯加钼组、顺铂加钼组,后3组均采用不同的浓度进行试验.用MTT法检测各组对人食管癌细胞ECA-109的生长抑制作用;流式细胞仪检测各组p75NTR百分率的变化.结果:顺铂组各浓度对食管癌细胞ECA-109有一定的抑制作用,且对食管癌干细胞也有不同程度的抑制作用,并且随着给药浓度和时间的增加均呈增强趋势;单纯加钼组对食管癌细胞ECA-109和对食管癌干细胞的抑制作用均不明显;顺铂加钼组对食管癌细胞ECA-109和食管癌干细胞的抑制作用则明显增强,与单用同浓度顺铂组及空白对照组比较有差异性(P<0.05),且呈一定的浓度、时间依赖性.结论:钼可明显增强顺铂对食管癌细胞ECA-109和食管癌干细胞的抑制作用,而单用钼则达不到理想的效果,说明钼可作为化疗增敏剂,为其作为食管癌化疗的辅助剂提供了实验依据.

AIM：To investigate the effect of molybdenum on the chemosensitivity of esophageal cancer ECA-109 cells to cisplatin and expression of p75NTR in esophageal stem cells.METHODS：ECA-109 cells were divided into four groups：blank control group,cisplatin group,molybdenum group,cisplatin plus molybdenum group.Cell proliferation was measured by methyl thiazolyl tetrazolium（MTT） assay.The expression of p75NTR in human esophageal stem cells was examined by flow cytometry.RESULTS：Cisplatin reduced the percentage of p75NTR-positive cells and the proliferation of ECA-109 cells in a dose-dependent manner.Treatment with molybdenum alone had no significant influence on the proliferation of ECA-109 cells and the percentage of p75NTRpositive cells.However,treatment with molybdenum significantly enhanced the chemosensitivity of esophageal cancer ECA-109 cells to cisplatin and cisplatin-induced inhibition of p75NTR expression in esophageal stem cells when compared to cells incubated with cisplatin alone or blank control cells（all P 0.05）.CONCLUSION：Molybdenum can increase the inhibitory effect of cisplatin on the proliferation of ECA-109 cells,which provides an experimental basis for the use of molybdenum as adjuvant chemotherapeutic agent for esophageal cancer.