膀胱癌组织中血管内皮生长因子的表达及与血管生成的关系

Expression of VEGF and tumor angiogenesis in bladder cancer

目的 探讨血管内皮生长因子 (VEGF)在原发性膀胱移行细胞癌中的表达及其与膀胱癌血管生成之间的关系。 方法 应用免疫组织化学技术检测 6 8例原发性膀胱移行细胞癌和 7例正常膀胱组织中血管内皮生长因子的表达 ,测定 40例浸润性膀胱癌的微血管密度 (microvesseldensity ,MVD) ,并分析VEGF和MVD间以及它们与膀胱癌病理分级和临床分期间的关系。 结果 VEGF在正常膀胱组织中不表达或低表达 ,而在膀胱癌组织表达较强 (中度以上表达占 6 4 7% ) ;VEGF表达及MVD值均与肿瘤的病理分级和临床分期密切相关 ,并且VEGF高表达者的MVD均值显著高于VEGF低表达者 (P <0 0 5 )。 结论 VEGF与膀胱癌的生长、浸润密切相关 ,是膀胱癌主要的血管新生诱导因子之一 ,可促进膀胱癌的血管生成。对膀胱癌组织中VEGF的测定将可能成为膀胱癌预后的一个有用指标 ,以用于识别高危转移和预后不佳者。

Objective[WT5”BZ] To evaluate expression of vascular endothelial growth factor (VEGF) and its relation to angiogenesis in bladder cancer. [WT5”HZ]Methods[WT5”BZ] The expression of VEGF by immunohistochemical staining was examined in 68 cases of primary transitional carcinoma (TCC) and 7 subjects with normal urothelium. Microvessel density (MVD) in 40 cases of invasive bladder cancer was determined by Factor Ⅷ immunohistochemical staining. [WT5”HZ]Results[WT5”BZ] The expression of VEGF was negative or low level in all normal bladder tissue but high in bladder cancer. VEGF and MVD were closely related to tumor cell nuclear grade and clinical stage. The average MVD was higher in the cases with the high level expression of VEGF, than in those with the low level expression of VEGF. [WT5”HZ]Conclusions[WT5”BZ] This study indicates that VEGF is positively correlated with the occurrence and progression of bladder cancer. VEGF is one of the primary angiogenic factors for bladder cancer, and is able to induce tumor angiogenesis and accelerate tumor growth. Quantification of VEGF may provide a valuable marker for a poorer prognosis of patients. [WT5”HZ]