摘要
目的 探讨胆固醇结石病与载脂蛋白 (Apo)B基因多态性的关系。 方法 选择载脂蛋白B基因多态位点XbaⅠ、EcoRⅠ应用聚合酶链反应和限制性片段长度多态性 (PCR RFLP)技术对 10 1例胆固醇结石病人和 5 0例对照者进行研究。结果 胆固醇结石组X+等位基因频率显著高于对照组 (P<0 .0 5 ) ,具有X+等位基因者其血脂总胆固醇 (TC)、低密度脂蛋白胆固醇 (LDL c)及ApoB水平显著高于非X+者 (P <0 .0 1) ;胆固醇结石组和对照组在EcoRⅠ等位基因频率和血脂水平方面差异均无显著性 (P >0 .0 5 )。结论 X+等位基因可能是胆固醇结石的易感基因。
Objective\ To investigate the relationship between the apolipoprotein (Apo)B gene polymorphic sites Xbal, EcoRI and cholesterol gallstone disease. Methods Two polymorphic sites Xbal, EcoRI of ApoB gene were selected. Using polymerase chain reaction and restriction fragment length polymorphism technique 101 patients with cholesterol gallstone and 50 controls were investigated. Results The frequency of rare allele X + was significantly higher in the patients with cholesterol gallstone than in the controls. Patients with X+ allele had higher TC, LDL-c and ApoB levels, significantly different from those without X+ allele. There were no difference between the patient group and the control one in EcoRI allele. Conclusion X+ allele might be the susceptible gene for cholesterol gallstone disease.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2000年第1期26-27,共2页
Chinese Journal of Experimental Surgery
基金
上海市科技发展基金!( 95 41190 2 7)