豚鼠体内生长抑素和血管活性肠肽在胆囊结石形成中的作用

Study on somatostatin and vasoactive intestinal peptide in guinea pig during gallstone formation

目的 探讨体内生长抑素 (somatostatin,SS)和血管活性肠肽 (vasoactiveintestinalpeptide,VIP)在胆囊结石形成中的作用。方法 采用放射免疫分析法 (RIA)和受体放射配基结合分析法 (RBA)分别测定豚鼠体内门静脉血浆、胆囊壁组织、胆汁中的SS、VIP和胆囊壁上VIP受体 (VIP R)。与对照组比较观察在诱发豚鼠胆囊结石实验的第 2、6、8周后胆囊空腹体积 (FV)、胆囊空腹胆汁量 (FB)、胆汁成分及上述胃肠肽的变化。结果 实验 2周后FV增大 ,6周和 8周后FB亦增大 ,胆汁发生致石性改变。在整个实验过程 ,豚鼠体内的SS均增高 ,胆汁中的VIP和胆囊壁上的VIP R最大结合容量 (Bmax)值增大。结论 体内SS和VIP等抑制性胃肠肽调节紊乱 ,诱发胆道生理功能失常 ,是胆囊结石形成的重要原因。

Objective\ To explore the role of somatostatin (SS) and vasoactive intestinal peptide (VIP) in gallstone formation.Methods\ Levels of SS and VIP in portal vein plasma, in gallbladder wall tissue and in bile of guinea pig were measured by using radioimmunoassay (RIA), and the values of Bmax and KD of VIP receptor (VIP R) in gallbladder wall tissue of guinea pigs determined by radioligand binding assay of receptor (RBA). Gallbladder fasting volume (FV), gallbladder fasting bile (FB), bile composition, and the results of the RIA and the RBA between the normal control group and the lithogenic group were compared respectively.Results\ The FV in guinea pigs was increased 2 weeks after lithogenic experiment. The FB was elevated and the bile become lithogenic 6 weeks and 8 weeks after experiment. The levels of SS in all the tissues, VIP in bile, and the value of IVP R Bmax were increased remarkably during the experiment period.Conclusion\ The disorder of SS and VIP regulation, which induces gallbladder function disorder, plays an important role in gallstone formation.