异种肝细胞移植治疗大鼠药物性肝衰的疗效观察

Therapeutic effects of xenogenic hepatocyte transplantation in the treatment of rat toxic fulminant hepatic failure

目的 观察异种肝细胞移植治疗大鼠药物性肝衰的疗效。方法 杂种豚鼠为供体 ,胶原酶消化法制备肝细胞。SD大鼠为受体 ,氨基半乳糖 (D GI)腹腔内 1次注射制作肝衰模型。 4 8h后将豚鼠肝细胞(1.5× 10 7个 )移植于大鼠脾内。同种移植及生理盐水为对照。移植后观察受体 2周存活率 ,在移植不同时间作移植物病理及组织化学检查。结果 受体 2周存活率 :异种移植组为 71% ,同种组为69 % (P >0 .0 5 ) ,生理盐水对照组为 2 5 % (P>0 .0 1)。豚鼠肝细胞移植后 12～ 2 4h其结构和功能基本保存完好。结论 与同种移植一样 ,异种肝细胞移植能逆转大鼠药物性肝衰。

Objective To observe the efficacy of xenogenic hepatocyte transplantation (XHT) in the treatment of toxic fulminant hepatic failure (FHF) in rat. Methods Fourteen SD rats received intraspleen graft of 1.5×10 7 guinea pig hepatocytes at 48 h after D glactosamine (D GI) intraperitonear injection (19.5 mg/kg). The survival rate for two weeks was compared with syngenic hepatocyte transplantation (SHT, n=16) and control animals (n=16). The pathological examination was carried out at different time points after transplantation. Results There was no significant difference in survival rate for 2 weeks between the XHT group and SHT group (71 % versus 69 %, P>0.05), but the survival rate in the control animals were significantly decreased (25 %, P<0.01). The anatomic structure and function of guinea pig hepatocytes transplanted were basically normal 12 to 24 h after transplantation.Conclusion XHT could reverse the rat FHF induced by D GI.