当归对氧化低密度脂蛋白致内皮细胞分泌一氧化氮下降和细胞间粘附分子-I表达升高的影响

Effects of Angelica Sinensis on OLDL-induced decrease of NO release from human endothelial cells and increase of ICAM-1 expression on the surface of human endothelial cells

目的 :探讨当归对氧化低密度脂蛋白 (OLDL)致血管内皮细胞 (VEC)损伤的细胞保护作用。方法 :以人脐静脉内皮细胞 (HUVEC)为实验模型 ,采用细胞培养 ,免疫组化等技术观察OLDL对VEC分泌一氧化氮(NO)及细胞间粘附分子 - 1(ICAM - 1)表达的影响并研究当归的保护作用。结果 :OLDL使EC分泌的NO显著降低 ,ICAM - 1的表达显著升高。当归明显拮抗这一作用 ,而阿托品能阻断当归的作用。结论 :当归能拮抗OLDL致内皮细胞分泌NO下降和ICAM - 1表达升高 。

AIM:To investigate the injurious effects of oxidized low-density(OLDL) on human endothelial cells(ECs) and protective effects of Angelica Sinensis in vitro. METHODS: The effect of OLDL on nitric oxide (NO) release from EC and intercellular adhesion molecule-1 (ICAM-1) expression on EC surface were studied, and the protective effects of Angelica Sinensis on normal and abnormal EC were investigated using cultured EC with or without OLDL and immunocytochemical staining technique. RESULTS: OLDL led to a decrease of NO release and an increase of ICAM-1 expression, which can be reversed by Angelica Sinensis and atropine can block this role of Angelica Sinensis. CONCLUSION: Angelica Sinensis has antagonistic effect on OLDL-induced decreasing of NO released from ECs and increasing of ICAM-1 expression on ECs surface . These effects of Angelica Sinensis may be exerted by the excitation of muscarinic receptor.