糖皮质激素受体减少对严重烫伤大鼠体内炎症介质水平的影响

The effect of glucocorticoid receptor on level of inflammatory media in rats after critical scald

目的 :阐明糖皮质激素受体 (GR)减少对严重烫伤大鼠体内几种炎症介质水平的影响。方法 :以RU38486阻断严重烫伤大鼠体内的GR ,观察其血浆磷脂酶A2 (PLA2 )活性、肿瘤坏死因子α(TNFα)和丙二醛(MDA)含量以及脏器组织匀浆中PLA2 活性和MDA含量变化。结果 :烫伤后 8h ,大鼠血浆及肺、肾组织匀浆中PLA2 活性、MDA和TNF含量均明显高于假处理对照组 (P <0 0 1) ;烫伤复合GR阻断组以上效应更显著 (P <0 0 1,P <0 0 5 )。结论 :烫伤复合GR阻断可导致大鼠体内炎症介质释放增多 。

AIM:To further elaborate the effect of glucocorticoid receptor(GR) decrease on level of inflammatory media in rats after critical scald.METHOD:The changes of phospholipase A 2(PLA 2) activity and concentrations of tumor necrosis factor alpha (TNFα) and malondialdehyde (MDA), the product of lipid peroxidation metabolism in plasma and tissue homogenate have been studied in scalded rats with or without GR blockade by RU38486.RESULTS:The PLA 2 activity and the concentrations of TNFα and MDA in plasma and homogenate of pulmonary and renal tissue in scalded rats were significantly higher than those in the controls( P <0.01) and further increased in scalded rats accompanied with GR blockade ( P <0.01 or P< 0 05).CONCLUSION:Blockade of GR might aggravate the increase of inflammatory mediator caused by scalding. It suggested that GR might potentiate anti-inflammation in scald.