转AlNHX1基因大豆后代遗传稳定性及耐盐性分析

Genetic stability analysis and salt tolerance of the progenies of soybean transformed AlNHX1 gene

为了获得耐盐性有所提高的转AlNHX1基因大豆后代材料,以已获得的转AlNHX1基因的6个株系中的3个株系后代为研究对象,通过分别对这3个株系转基因大豆各后代进行PCR分子检测并结合耐盐性鉴定,以分析外源基因在转基因大豆中遗传稳定性和耐盐性.结果表明:AlNHX1基因在转基因植株的后代中遗传;选取3个株系中部分阳性植株做耐盐性检测,结果表明:转基因大豆耐盐性状均好于野生型大豆.在150mmol/L NaCl胁迫下,转基因大豆叶片中维持了相对较高的K+/Na+比值,相对含水量较野生型提高了9%,而渗透势降低了39%,表明转基因大豆具有较好的吸水和保水能力;在盐胁迫下,超氧化物歧化酶(SOD)与过氧化物酶(POD)活性较野生型大豆分别提高了45%与69%.综上,通过耐盐筛选获得的转AlNHX1基因大豆具有较强的耐盐性.

To obtain the progenies of AlNHXl-expressing soybean with increasing salt-tolerant capac- ity, three of six transgenic soybean lines and their progenies which introduced the Na＋/H＋ antiporter gene （AlNHX1） were used as materials to perform the genetic stability, analyse their resistance to salt stress. The results showed that the AINHX1 gene could inherit steadily in transgenic soybean plants. Under 150 mmol/L NaC1 salt stresses, a higher ratio of K^＋ to Na^＋ was retaining in the leaves of the transgenic plants. The osmotic potential in the leaves of the transgenic plants decreased by 39 % and relative water in- creased by 9%. Transgenic plants also indicated an increase in SOD （a.5%） and POD （69%） activities over wild-type plants. The results demonstrated that the AINHX1 gene was strongly expressed in soybean, with the increasing its salt-tolerant capacity under 150 mmol/L NaC1 salt stress.