乌头碱羟基琥珀酰亚胺酯及其BSA结合物的制备

The Preparation of Aconitine N-Hydroxysuccinimide Esters and Their Reactivities with Bovine Serum Albumin

为建立乌头碱酶免疫测定法，合成了乌头碱衍生物，用羟基琥珀酰亚胺酯的方法制备半抗原（ 活性酯） ，并用其半抗原与ＢＳＡ（Ｂｏｖｉｎｅ Ｓｅｒｕｍ Ａｌｂｕｍｉｎ） 在有机溶媒／ 磷酸缓冲液（ｐＨ７－３） 的条件下制备了抗原性ＢＳＡ 结合物．结果显示，这种活性酯的方法可用于乌头碱酶免疫测定法中与ＢＳＡ 或酶交联的有效方法．

In order to establish the enzyme immunoassay of aconitine,aconitine derivatives were synthesized,which is called the chemical bridge.The preparation of the hapten of aconitine was carried out by the N hydroxysuccinimide esters method.These activated esters were covalently linked to BSA(Bovine Serum Albunin)in an organic solvent/phosphate buffer(pH 7 3) employing 1∶30 molar ratio of the aconitine to protein.The results indicated that the activated ester method would be useful for enzyme labeling in enzyme immunoassay of aconitine in respect of simplicity and reproducibility.