漳州水仙矮化机理研究

Dwarfing mechanism of Narcissus tazetta L. var. chinensis Roem treated with PP333

【目的】在前面研究基础上,以最佳多效唑浓度对水仙进行矮化处理,探讨多效唑调控水仙植株矮化的机理。【方法】以漳州水仙为材料,以40mg/L多效唑(PP333)对水仙进行矮化处理,研究其对水仙植株体内氮、碳代谢的影响。【结果】经40mg/LPP333矮化处理后,不同时期水仙叶片硝态氮、铵态氮含量呈不同程度降低,而硝态氮与铵态氮比值增大,抽苔期前叶片总氮含量也有所增加;叶片总糖和还原糖含量、还原糖占总糖比例均有不同程度提高,而蔗糖含量较清水对照表现出不规则变化。【结论】PP333矮化处理使水仙叶片氮、碳代谢发生明显改变,氮素含量及其形态的变化与水仙植株的矮化关系密切,总糖和还原糖含量与水仙植株矮化的关系较蔗糖含量与其的关系更为明显。

[Objective]On the basis of initial studies,the dosage of PP333 were screened and optimized for Narcissus to study the mechanism of dwarfing regulation in Narcissus.[Method]Narcissus tazetta L.var.chinensis Roem sampled from Zhangzhou was treated with 40 mg/L PP333 to investigate the nitrogen and carbon metabolism in leaf.[Result]The PP333 treatment decreased the inorganic nitrogen（ammonium and nitrate nitrogen）,increased the ratio of NO3-N to NH4-N in leaf,as well the total nitrogen content before bolting stage.The total soluble sugar and reducing sugar in leaf,and percentage of reducing sugar in total sugar also increased.The sucrose content in leaves of PP333 treated plants did not show regular changes compared to the control.[Conclusion]Treatment of 40 mg/L PP333 changed the carbon and nitrogen metabolism of dwarf Narcissus significantly.The nitrogen content and changes in its form were closely related to Narcissus dwarfing,and the total sugar and reducing sugar content were found more closely related to Narcissus dwarfing than sucrose content.