菌株GLV-85产赤霉素固体发酵条件的研究

Optimizing the Gibberellin Solid Fermentation Conditions of Gibberella Fujikuroi GLV-85

以赤霉素产量为测定指标,采用单因素及正交试验对藤仓赤霉菌GLV-85菌株固体发酵赤霉素的培养基及培养条件进行了优化。获得固体发酵的最佳培养基为:在250ml三角瓶中装入10g固体基质,其中麸皮:玉米面按4:6混合,MgSO4含量0.03g,KH2PO4含量0.01g;最佳发酵条件为:接种量10%,发酵温度32℃,发酵pH7,固液比1:1.2,发酵周期7d。在最优的发酵条件下,赤霉素的发酵效价达到9.832g/kg(干基),较优化前提高了1.15倍。

The solid fermentation medium and culture conditions of gibberella fujikuroi GLV-85 were studied according to the gibberellin content by single factor and orthogonal experiments. The most optimal medium was obtained as follows： 250ml Erlenmeyer flask contained 20g of wheat bran and maize powder mixture with ratio of 4：6, MgSO4 0.03g, KH2PO4 0.01g. The most optimal culture conditions were inoculum volume 10%,fermentation temperature 32℃,fermentation pH 7,the ratio of material to water is 1：1.2, fermentation time 6d. Under the optimized conditions, gibberellin content was up to 9.832g/kg,it improved 115% than that of basic medium（4.573 g/kg）.