正义和反义Calpastatin cDNA真核表达载体的构建

THE CONSTRUCTION OF pRC/RSV SENSE AND ANTISENSE CALPASTATIN cDNA EXPRESSION VECTORS

本研究利用真核表达载体 pRC/RSV ,将位于 pBS SK质粒中的CalpastatincDNA以正反两个方向构入载体 ;经酶切分析 ,构建的质粒结构正确。所构建质粒 pRC/RSV正义、反义CalpastatincDNA两个表达载体 ,为进一步转化肌细胞 ,人为控制Calpastatin基因的表达 ,从而控制Calpain活性 ,深入研究钙蛋白酶在肌肉发育及肉嫩化中的作用打下基础。

WT5BZ] Calpain system has been suggested to play a key role in myoblast fusion and muscle growth.Possibly,we can enhance the growth of muscle through controlling the activity of calpains.Calpastatin is the endogenous inhibitor of calpains which regulate calpain activity in vivo .This research employs eukaryotic expression vector pRC/RSV and Calpastatin cDNA in pBS SK to construct two expression vectors in which calpastatin cDNA are inserted in sense and antisense direction respectively.Endonuclease restriction analysis proved that the two plasmids were correctly constructed.Those plasmids will be useful in regulating the activities of calpains by controlling the expression of calpastatin gene,and facilitate researches in the role of calpain system in muscle growth and meat tenderization. [WT5HZ]