摘要
AIM:To investigate whether high-fat-feeding is associated with increased intestinal permeability via alterations in bile acid metabolism.METHODS:Male C57Bl/6J mice were fed on a high-fat (n=26) or low-fat diet (n=24) for 15 wk.Intestinal permeability was measured from duodenum,jejunum,ileum and colon in an Ussing chamber system using 4 kDa FITC-labeled dextran as an indicator.Fecal bile acids were analyzed with gas chromatography.Segments of jejunum and colon were analyzed for the expression of farnesoid X receptor (FXR) and tumor necrosis factor (TNF).RESULTS:Intestinal permeability was significantly increased by high-fat feeding in jejunum (median 0.334 for control vs 0.393 for high-fat,P=0.03) and colon (0.335 for control vs 0.433 for high-fat,P=0.01),but not in duodenum or ileum.The concentration of nearly all identified bile acids was significantly increased by high-fat feeding (P<0.001).The proportion of ursodeoxycholic acid (UDCA) in all bile acids was decreased (1.4%±0.1% in high-fat vs 2.8%±0.3% in controls,P<0.01) and correlated inversely with intestinal permeability (r=-0.72,P=0.01).High-fat feeding also increased jejunal FXR expression,as well as TNF expression along the intestine,especially in the colon.CONCLUSION:High-fat-feeding increased intestinal permeability,perhaps by a mechanism related to bile acid metabolism,namely a decreased proportion of fecal UDCA and increased FXR expression.
AIM: To investigate whether high-fat-feeding is associ- ated with increased intestinal permeability via altera- tions in bile acid metabolism.
METHODS: Male C57BI/6J mice were fed on a high-fat (n = 26) or low-fat diet (n = 24) for 15 wk. Intestinal permeability was measured from duodenum, jejunum, ileum and colon in an Ussing chamber system using 4 kDa FITC-labeled dextran as an indicator. Fecal bile ac- ids were analyzed with gas chromatography. Segments of jejunum and colon were analyzed for the expression of farnesoid X receptor (FXR) and tumor necrosis factor
基金
Supported by The Foundation for Nutrition Research and the Finnish Funding Agency of Technology and Innovation
关键词
通透性
高脂肪
胆汁酸
功能障碍
肠道
粪便
ALTERA
气相色谱分析
Bile acids
Bile salts
Diet-induced obesity
Farnesoid X-activated receptor
Intestinal permeability
Ursodeoxycholic acid