甲壳素神经再生室注入聚乳酸-聚乙醇酸-重组人促红细胞生成素微球促进缺损周围神经的修复

Injection of polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres into chitin nerve regeneration chamber can promote sciatic nerve regeneration

背景:促红细胞生成素除了具有造血的作用以外,对神经系统损伤的修复也起着重要作用。目的:观察聚乳酸-聚乙醇酸-重组人促红细胞生成素微球对大鼠坐骨神经再生的作用。方法:雌性SD大鼠60只,随机分为3组。制备大鼠双侧坐骨神经缺损模型(1cm缺损)以及可吸收甲壳素神经再生室。实验组室内注入聚乳酸-聚乙醇酸-重组人促红细胞生成素微球;对照组室内注入聚乳酸-聚乙醇酸微球;空白对照组室内注入等渗生理盐水。结果与结论:实验组再生神经的传导速度优于对照组及空白对照组,且12周优于6周,差异有显著性意义(P<0.05)。S-100免疫组织化学及Loyez氏神经染色法显示:实验组神经纤维数量多于对照组及空白对照组,12周多于6周,差异有显著性意义(P<0.05)。结果提示聚乳酸-聚乙醇酸-重组人促红细胞生成素微球能够促进实验性坐骨神经缺损的再生和功能的恢复。

BACKGROUND：Erythropoietin（EPO） plays an important role in hematopoiesis as well as in the repair of nervous system injury.OBJECTIVE：To observe the effect of polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres on sciatic nerve regeneration.METHODS：Sixty female SD rats were randomly divided into three groups,20 rats in each group.The bilateral sciatic nerve defect model was prepared（1 cm defect） and the absorbable chitin nerve regeneration chamber was prepared.The chambers of the rats in the experimental group were injected with polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres,chambers of the rats in the control group were injected with polylactic acid-polyglycolic acid microspheres and chambers of the rats in the blank control group were injected with normal saline in the same dose.RESULTS AND CONCLUSION：The conduction velocity of the regeneration nerve in the experimental group was better than that in the control group and blank control group,and the conduction velocity at the 12th week was better than that at the 6th week.There was significant difference（P 0.05）.S-100 immunohistochemistry and Loyezs neural staining showed that the number of nerve fibers in experimental group was more that in control group and blank control group,and the number of nerve fibers at the 12th week was more than that at the 6th week;the difference was statistically significant（P 0.05）.Polylactic acid-polyglycolic acid-recombinant human erythropoietin microspheres has the effect of promoting sciatic nerve regeneration and functional recovery.