丝素蛋白/壳聚糖三维多孔支架的构建及结构表征

Preparation and characterization of porous silk fibroin/chitosan scaffolds

背景:丝素蛋白和壳聚糖均无毒性且具有良好的生物相容性,但是单一成分作为生物支架时都不能满足支架材料的需求。目的:制备各种不同组分的丝素蛋白及壳聚糖复合支架材料,观察其微观结构及相关性能,筛选出适合成骨细胞生长的理想支架材料。方法:通过CaCl2:C2H5OH:H2O=1:2:8(摩尔比)溶解体系溶解、过滤、浓缩提纯,制备出2%的丝素蛋白溶液,壳聚糖溶解于乙酸溶液配制成的3%壳聚糖溶液,将两者以不同的比例相混合,经数次冷冻干燥后,得到成品支架材料。采用电镜观察形貌,计算孔隙率并对支架的结构进行红外、X射线衍射、电子能谱分析观察。结果与结论:将壳聚糖和丝素蛋白共混后,互为改性,制备出了结构较稳定的支架材料。其中40%丝素蛋白-60%壳聚糖组具有适合成骨细胞生长的较佳孔径,可作为细胞支架的首选配比。

BACKGROUND：Silk fibroin and chitosan are non-toxic and have a good histocompatibility.However,they could not satisfy the biological scaffold material needs if working separately.OBJECTIVE：To explore and analyze the material structure and relate performance for characterization on the basis of making a variety of compound scaffolds with different proportion of silk fibroin and chitosan,and to select the ideal scaffolds that suitable for the growth of osteoblasts.METHODS：2% silk fibroin solution was made by dissolution from CaCl2 solvent systems at a mole ratio of CaCl2：C2H5OH：H2O= 1：2：8,filtration,concentration and purification.The chitosan was dissolved in acetic acid solution prepared 3% chitosan-acetic acid solution.The silk fibroin and chitosan were blend in a freeze-drying approach in different proportion in order to get different scaffold materials.Then the morphology of the silk fibroin/chitosan scaffolds was observed under a scanning electron microscope;the porosity was calculated and the structure of the scaffolds was evaluated and observed by Fourier transform infrared spectroscopy,X-ray diffraction and electron spectroscopy.RESULTS AND CONCLUSIONS：More stable scaffolds were created with the method of blending and modifying silk fibroin and chitosan.The 40% silk fibroin-60% chitosan group proves the preferred ratio of cell scaffold material due to its better aperture for the growth of osteoblasts.