脂联素对兔下颌快速牵张成骨的影响

Effect of adiponectin on bone regeneration following rapid mandibular distraction osteogenesis in rabbits

背景:脂联素可参与骨代谢及成血管过程,但目前关于脂联素对牵张成骨有无促进作用尚不清楚。目的:通过建立兔下颌快速牵张动物模型,探讨局部应用脂联素对骨牵张新骨再生的影响。方法:16只新西兰大白兔随机摸球法均分为对照组及实验组,建立兔下颌单侧快速牵张模型,牵张速率为2mm/d。在牵张开始的1,3,5d,对照组及实验组分别于牵张间隙注入200μL磷酸盐缓冲液或含有2μg重组人脂联素的磷酸盐缓冲液。结果与结论:两组动物牵张间隙内均可观察到新骨生成,组织学及显微CT检查显示实验组的新骨生成与钙化明显高于对照组。实验结果显示局部应用脂联素可有效促进兔下颌快速骨牵张的新骨再生。

BACKGROUND： Adiponectin participates in bone metabolism and angiogenesis, which is beneficial for bone growing. However, the effect of adiponectin on bone regeneration following distraction osteogenesis is not clear. OBJECTIVE： To investigate the effects of intermittent local administration of adiponectin on bone regeneration following establishment of rapid mandibular distraction models in rabbits. METHODS： Sixteen New Zealand white rabbits were randomly divided into experimental group and control group. Rapid unilateral mandibular distraction osteogenesis models were established with 2 mm/d distraction rate. At the 1, 3 and 5 days of the distraction, 200 μL of phosphate buffer solution or 200 μL of phosphate buffer solution containing 2 μg recombinant human adiponectin was intermittently injected into the distraction gap of the control and experimental groups, respectively. RESULTS AND CONCLUSION： Bone regeneration was found in the distracted callus from both groups. Histological observation and micro-CT examinations confirmed that the new bone formation and mineralization of the distraction callus in experimental group was significantly greater than that of the control group. Intermittent administration of adiponectin can markedly promote the bone regeneration in rabbit rapid mandibular osteodistraction.