肾间质纤维化模型大鼠转化生长因子β/Smads信号传导通路变化与温阳活血方的干预

Effects of Wenyang Huoxue recipe on transforming growth factor beta/Smad signal transduction pathway in model rats of renal interstitial fibrosis

背景:转化生长因子β/Smad信号转导通路在肾纤维化的发生发展中起重要作用。目的:探讨温阳活血方对肾纤维化转化生长因子β/Smads信号传导通路的影响。方法:选用清洁级雄性SD大鼠28只,随机分为假手术组和模型组,模型组行左侧输尿管结扎术,单侧输尿管梗阻模型。假手术组大鼠打开腹腔后分离左侧输尿管但不结扎,随即关闭腹腔。造模后第2天开始分别给予温阳活血方、血管紧张素转化酶抑制剂或生理盐水灌胃,1次/d,各组均连续灌胃14d。结果与结论:温阳活血组转化生长因子β1、Smads3的表达显著低于模型组(P<0.01),Smad7表达显著高于模型组(P<0.01),与福辛普利组相当。推测温阳活血方可能通过抑制转化生长因子β1、Smads3的表达,上调Smad7的表达,进而影响转化生长因子β/Smad信号传导通路,对肾间质纤维化起防治作用。

BACKGROUND： Transforming growth factor β/Smad signal transduction pathway plays an important role in the occurrence and development of renal fibrosis. OBJECTIVE： To investigate the effects of Wenyang Huoxue recipe on transforming growth factor β/Smad signal transduction pathway in renal interstitial fibrosis rats. METHODS： A total of 28 male SD rats of clean grade were randomly assigned into sham operation group and model group. Left urethral ligation was performed in rats to construct unilateral ureteral obstruction model. The left ureter of sham operation rats was isolated without ligation after opening the abdominal cavity; then the abdominal cavity was closed. The rats were administrated with Wenyang Huoxue recipe by gavage once. Rats in both groups were administered by gavage for 14 days. RESULTS AND CONCLUSION： The expression of transforming growth factor β1 and Smad3 in the Wenyang Huoxue group was significantly lower than that in the model group （P 0.01）. The expression of Smad7 in the Wenyang Huoxue group was significantly larger than that in the model group （P 0.01） and similar to that in the fosinopril group. It is conjectured that Wenyang Huoxue recipe may inhibit the expression of transforming growth factor β1 and Smad3, increase Smad7 expression, thereby affecting the transforming growth factor β/Smad signaling pathway, and thus play a role in the prevention and cure of renal interstitial fibrosis.