贯穿缝扎联合打孔法构建兔耳难愈性创面模型

Construction of a rabbit ear model of chronic wound using transfixion ligation combined punch method

背景:以往制作的兔耳难愈创面模型仅局限于单纯的缺血模型,缺血淤血模型少见。目的:建立一种方法简单、效果确实的兔耳难愈性创面模型。方法:将40个兔耳分为两组:实验组贯穿缝扎兔耳根部背腹侧中央、头侧所有动、静脉,缝扎后第10天在耳背侧用活检打孔器制作3个深达软骨面的圆形创面。对照组仅制作与实验组完全相同的3个圆形创面。结果与结论:实验组兔耳缝扎后立即呈现紫黑色,第10,20,30天为淡紫黑色、紫色、淡紫色,说明贯穿缝扎联合打孔法构建的难愈创面的缺血、淤血状态持久、稳定。实验组创面肉芽组织生长、表皮化时间及创面愈合时间均较长于对照组均延迟(P<0.01),血管内皮细胞生长因子表达明显低于对照组(P<0.01)。说明贯穿缝扎联合打孔法制作的兔耳难愈性创面模型方法简单、效果确实可靠,可作为缺血淤血因素所致难愈性创面修复的实验研究模型。

BACKGROUND： In the past, the constructed rabbit ear models of chronic wound are limited to simple models of ischemia, while the model of both ischemia and congestion has been rarely reported. OBJECTIVE： To establish a chronic wound model for ischemia and congestion using rabbit ears. METHODS： A total of 40 rabbit ears were divided into two groups, experiment group and control group. The area of the dorsoventral side in ear root, all cephalic and center arteries and veins were transfixion ligated in the experiment group. Three circular wounds as deep as the cartilage surface were punched at the dorsal sides of the ears, using biopsy punch on the 10th day after ligation. Three circular wounds identical to that of the experiment group were punched in the control group. RESULTS AND CONCLUSION： The color of rabbit ears in the experiment group was deep violet-black immediately after ligation, and was somewhat violet-black, violet and lilac on the 10th, 20th and 30th days after ligation. It illustrates that the status of ischemia and congestion is durable and stable in the chronic wound model constructed using transfixion ligation combined punch method. Compared with the control group, the time of granulation tissue growth, the time of wound healing and epidermidalization in the experiment group were obviously delayed （P 0.01）. The expression of vascular endothelial growth factor in the experiment group was obviously lower than that of the control group （P 0.01）.These findings demonstrate that rabbit ear models of chronic wound constructed using transfixion ligation combined punch method are simple and reliable. It can be used as an ideal animal study model in the research of chronic wounds repair caused by ischemia and congestion factors.