雄性大鼠长期有氧运动期间血液铁状态与饮食铁含量的关系

Effect of dietary iron contents on hematological iron status in male rats with long-term aerobic exercise

背景:运动对雌性大鼠血液铁状态的影响也已经得到广泛研究,但运动对雄性大鼠血液铁状态的影响仍不明确。目的:观察不同铁含量饮食以及游泳运动对雄性大鼠血液铁状态的影响。方法:断乳雄性SD大鼠90只,分为饮食低铁含量组、标准铁含量组、高铁含量组。每组再分为运动组和静息组。用不同的铁含量饲料喂养1个月后,运动组开始游泳,每天1次,持续3个月,静息组除不做运动外,其余处理同对应运动组。最后1次运动后,大鼠空腹24h,戊巴比妥钠麻醉下取静脉血测定红细胞相关指标和血清铁状态指标。结果与结论:饮食铁含量对红细胞和血清铁状态指标的主效应都有显著影响,运动对红细胞分布宽度、血浆总铁结合力的主效应也显著影响。饮食低铁含量静息组表现为铁缺乏性贫血的典型改变,而运动组血清铁和转铁蛋白饱和度显著降低,血浆总铁结合力显著增大,说明低铁饮食情况下运动加重了血液低铁状态。饮食标准铁含量、高铁含量的运动组都表现为红细胞分布宽度显著增大,血浆总铁结合力显著增大,但其他指标均无显著改变,说明运动没有导致血液低铁状态。

BACKGROUND： Effects of exercise on hematological iron status have been extensively studied in female rats, but those effects have not been observed in male rats. OBJECTIVE： To investigate the effects of dietary iron contents and swimming exercise on hematological iron status of male rats. METHODS： Ninety weaning male rats were assigned into three groups： dietary iron deficiency group, dietary iron sufficiency group and dietary high iron content group, and then each group was further divided into exercise group and sedentary group. After one month of feeding with different iron contents diet, the rats in each exercise group entered a swimming program once a day, lasting for 3 months, and the rats in the corresponding sedentary groups received approximately the same amount of handling as the exercised rats, except for swimming. Animals were fasted for 24 hours after the last exercise regimen and then blood collection was taken from their hearts under pentobarbital anesthesia for analyzing related index of red blood cells and total iron binding capacity （TIBC）. RESULTS AND CONCLUSION： Dietary iron contents could significantly affect the main effects of index of red blood and TIBC, and exercise could significantly affect the main effect of the red cell distribution and TIBC. In the dietary low iron content groups, the sedentary rats had the changes of iron deficiency with anemia, but the exercised rats had the significantly lower serum iron and transferrin saturation and the significantly higher TIBC. It showed that exercise under the condition of dietary iron deficiency could deteriorate serum iron status. Both in the dietary sufficient iron group and the dietary high iron content group, exercise could significantly increase red cell distribution and TIBC with no significant changes of other red blood cell indexes and serum iron, it suggested that exercise under the condition of dietary sufficient iron content may not induce the hematological low iron status.