富集骨髓间充质干细胞用于兔腰椎横突间的融合

Enrichment of bone marrow stromal stem cells for lumbar intertransverse spinal fusion of rabbits

背景:纳米羟基磷灰石/胶原具有良好的细胞相容性,其纳米级多孔结构可为细胞生长创造适合的三维环境,可作为理想的骨载体材料。目的:探讨富集骨髓间充质干细胞复合纳米羟基磷灰石/胶原用于兔腰椎横突间融合的可行性。方法:20只新西兰大白兔根据双侧L5～L6横突间植入物不同分为富集细胞组和自体髂骨组,每组10只。植入后8周取材,行X射线、手触检测和组织学观察,评估腰椎融合情况。结果与结论:植入后8周富集细胞组和自体髂骨组融合率分别为70%(7/10)和80%(8/10),两组融合率差异无显著性意义(P>0.05)。组织学观察富集细胞组见较多新生骨小梁长入纳米羟基磷灰石/胶原内部;自体髂骨组横突间有大量新生骨组织,骨小梁连续。结果表明富集骨髓间充质干细胞复合纳米羟基磷灰石/胶原是一种较好的植骨替代材料,用于脊柱融合可获得近似自体骨移植的融合效果。

BACKGROUND：Nano-hydroxyapatite/collagen has good cellular compatibility.It can create three-dimensional environment for cells growth and be well used as bone carrier material based on its nano-porous structure.OBJECTIVE：To explore the feasibility of the enriched bone marrow stromal stem cells（BMSCs） composite with nano-hydroxyapatite/collagen（nHAC） for lumbar intertransverse spinal fusion of rabbits.METHODS：Twenty New Zealand rabbits were divided into the cells group（n=10） and the autograft group（n=10） according to their different implants in bilateral L5-L6 intertransverse process.Drawing materials was performed 8 weeks after implantation.Then the technique detection and histological observation were performed in order to estimate the lumbar fusion.RESULTS AND CONCLUSIONS：The fusion rates in the cells group and the autograft group were 70%（7/10） and 80%（8/10） respectively 8 weeks after implantation,and had no significant difference（P 0.05）.Histological observation showed that there was more newly formed bone trabeculae growth inside the nHAC of the cells group.A great amount of new bone tissues and linked bone trabeculae were seen in intertransverse process of the autograft group.This study showed that enriched BMSCs composite with nHAC can be used as a substitute for autograft in spine fusion,and is similar to autograft in spinal fusion.