CyclinE和cdk2在眼睑基底细胞癌组织中的表达及其意义

Expression and significance of Cyclin E and Cdk2 in eyelid basal cell carcinoma

目的探讨CyclinE和cdk2在眼睑基底细胞癌组织中的表达及其意义。方法收集武汉市中心医院和武汉大学人民医院病理科2002-2009年手术切除及活检的眼睑基底细胞癌(basal cell carcinoma,BCC)标本共20例,另取癌周围组织5例作对照。采用免疫组织化学方法观察各组细胞内CyclinE和cdk2表达。利用HPIAS-2000图像分析系统测定CyclinE和cdk2在以上各组中表达的平均光密度和平均阳性面积率。结果眼睑基底细胞癌组织中CyclinE和cdk2呈高表达;癌旁组织中CyclinE和cdk2呈低表达。图像分析结果显示:眼睑基底细胞癌组织与癌旁组织之间CyclinE和cdk2的平均光密度及阳性面积率的差异有显著性意义(P<0.05)。结论 CyclinE和cdk2的高表达,在眼睑基底细胞癌的发生、发展过程中起了重要作用,两者共同表达对于术后病人监测及治疗方案的制定具有指导意义。

Objective To research the expression and significance of CyclinE and cdk2 in basal cell carcinoma of eyelids. Methods Twenty samples of eyelid basal cell carcinoma（BCC） from surgical excision and biopsy were collected from the departments of pathology of Wuhan Central Hospital and Wuhan University Zhongnan Hospital in 2002-2009,and 5 normal tissues around the cancer acted as controls.Immunohistochemical staining was performed to detect the expression of Cyclin E and Cdk2.The average optical density and the rate of positive area of Cyclin E and Cdk2 expression were analyzed using the HPIAS-2000 Image Analysis System. Results Cyclin E and Cdk2 showed high expression in eyelid basal cell carcinoma,but low expression in paracancerous tissue.Image analysis showed that the expression of CyclinE and cdk2 in eyelid basal cell carcinoma were significantly higher than that in paracancerous tissue（P0.05）. Conclusion High expression of Cyclin E and Cdk2 plays an important role in the occurrence and development of eyelid basal cell carcinoma,and their co-expression is of guiding significance and for formulating therapeutic plans and for monitoring patients after treatment.