11个毛木耳主栽菌株的鉴别研究

The Identification Research of 11 Auricularia polytricha Mont.Sacc Strains Cultivated

为了对实验室保藏的11个毛木耳品种进行菌株鉴别,以期减少实际生产中的同种异名现象,为指导生产提供依据。利用拮抗试验和酯酶同工酶鉴定法对不同的毛木耳栽培菌株进行鉴别。结果表明,2种方法得到了一致的结论。11个菌株分为6类,第1类包括毛木耳3号等4个菌株;第2类包括白背120等3个菌株;其他菌株各分属不同类别。通过研究认为,在食用菌菌种的鉴别中,应用拮抗实验对供试菌株进行初步筛选是必要的,可以减少工作量和盲目性,为进一步的研究提供依据。同时酯酶同工酶鉴定法从分子方面验证了菌株类别,为遗传学研究和育种提供了依据。

The aim of this research was to distinguish 11 cultivated strains of Auricularia polytricha Mont. They were analyzed by antagonistic test and esterase isoenzyme,The results showed that the two methods got the same conclusions,and 11 strains could be divided into six groups：the first group including 4 strains of Auricularia polytricha Mont.No.3 etc.the second group including 3 strains of white back 120 etc.The others belonged to different groups.Therefore,in order to reduce the workload and blindness,and to provide a basis for further research,it was necessary to utilize antagonistic test for a preliminary screening on tested strains in the identification of edible fungi strains.Esterase isoenzyme was also effective for the classification of strain. The results could be comprehensively analyzed for providing the basis in genetics research and mushroom breeding.