蛋白磷酸酶Ⅰ(PPP1R3)在直肠癌细胞体外侵袭转移的生物学活性研究

THE STUDY ON BIOLOGICAL ACTIVITY WITH INVASION AND METASTASIS OFVITRO RECTAL CANCER CELL BY PPP1R3

目的探讨研究蛋白磷酸酶I(protein phosphatase1 regulatory subunit 3,PPP1R3)在直肠癌SW480细胞中的黏附、运动、迁移和侵袭能力的生物学活性。方法用PPP1R3、EGFR抗体、VEGFR抗体干预SW480细胞后,分别对其行细胞运动实验、黏附实验、迁移及侵袭实验,分析PPP1R3对SW480细胞运动、黏附、迁移和侵袭能力的影响及其作用机制。结果随着PPP1R3浓度的增加,直肠癌SW480细胞的黏附、运动、迁移和侵袭能力明显减退(P<0.05)。而且穿过人工基底膜的能力明显减低(P<0.05)。结论 PPP1R3能够抑制直肠癌SW480体外的侵袭、转移作用,可为PPP1R3基因表达缺失的直肠癌术后患者提供放化疗、生物靶向治疗依据。

Objective To investigate whether the PPP1R3 enhances adherence,movement,invasion and metastasia capabilitier of rectal cancer cell（SW480）. Methods Biological activities, such as adherence, movement,invasion and metastasia of SW480 cells ,were treatde by PPP1R3, EGFRantibody, VEGFRantibody, and the mechanism was investigated. Results The biological activities such as adherence, movement, invasion,metastasia and crossing the reconstituted basement membrane were failed with the increased con- centration of PPP1R3. Conclusion The abilities of vitro rectal cancer such as membrane,invasion are inhabited by PPP1R3. It can supply the evidence of chemotherapy and targeted biological therapy for the post- operative patients of Rectal cancer surgery with PPP1R3 gene deletion.