摘要
目的探讨急性一氧化碳中毒对大鼠脑红蛋白的影响,为一氧化碳中毒的防治提供理论依据。方法健康雄性SD大鼠20只随机分为对照组和一氧化碳染毒组,每组10只。染毒组按60ml/kg体重腹腔注射一氧化碳,每周一次,注射4周。对照组注射等量的空气。染毒结束后进行神经行为学测试包括旷场试验、甩尾试验和后肢撑力试验,同时检测大鼠海马、皮质、小脑中脑红蛋白的变化。结果一氧化碳中毒后大鼠在中央格停留时间明显增加,修饰次数、直立次数和大小便次数也较对照组明显增加,穿格次数明显减少;甩尾时间比对照组明显延长、后肢展开距离增加明显,差异有统计学意义。此外CO染毒大鼠皮质和小脑中的NGB含量增加,而海马中的NGB呈下降的趋势。结论一氧化碳中毒致脑组织脑红蛋白含量变化,这可能参与了CO中毒致神经损伤的机制。
Objective To investigate the damage of acute carbon monoxide(CO)poisoning on rats' neuroglobin(NGB), in order to provide a theoretical basis for the preventions and treatments to CO poisoning. Methods 20 healthy male SD rats were randomly divided into control group and Carbon monoxide exposure group, and each for 10 rats. The poisoned group was abdominal injected with Carbon monoxide at 60ml/kg (once a week )for four weeks. The control group was injected with the same volume of air. After Carbon monoxide poisoned, neurobehavioral tests were performed including open- field test, tail- flick and hind legs to hold power test. Meanwhile the contents of neuroglobin were measured in hippocampus,cortex, cerebellum. Results The staying time oS in the central grid Carbon monoxide poisoned rats was obviously increased. The frequency of modify, upright, defecate and urinate were also visibly higher compare with the control group. The frequency of translocation between grids was significantly lessened, and the tail- flick latencies and the expansion distance of hind limbs visibly increased than that of control group. In addition, compare with control group, the NGB content in the cortex and cerebellum of poisoned group increased, on the contray, the NGB in the hippocampus showed a decreasing trend. Conclusion Carbon monoxide poisoning changes the neuroglobin content in brain tissue, which might involved in the mechanism of Carbon monoxide poisoning induced central nerve system injury.
出处
《中国煤炭工业医学杂志》
2012年第4期571-573,共3页
Chinese Journal of Coal Industry Medicine
基金
卫生部卫生行业科研专项(200902006)
河北省教育厅11276104D