凉血活血胶囊对咪喹莫特诱导小鼠银屑病样皮损的干预作用

Effect of Liang Xue Huo Xue capsules on mouse psoriasis-like lesions induced by imiquimod

目的:观察凉血活血胶囊对咪喹莫特诱导银屑病样小鼠模型中免疫异常的干预作用。方法:BALB/c雌性小鼠48只,随机分为正常对照组,模型组,凉血活血胶囊高、中、低剂量组和雷公藤多苷组。采用银屑病皮损面积和疾病严重程度(psoriasis area and severity index,PASI)评分标准观察银屑病样小鼠模型皮损变化情况。光镜下观察皮损组织形态学变化,测量表皮层厚度。免疫组织化学法检测皮损中增殖细胞核抗原(proliferatingcell nuclear antigen,PCNA)反映表皮角质形成细胞增殖程度;检测CD3、CD11c、F4/80和Gr-1反映炎症浸润程度;检测CD31以反映血管增生情况。结果:模型组小鼠皮肤出现鳞屑红斑,皮损增厚;皮损组织表现为表皮棘层增厚,角化不全和微脓肿;真皮大量炎症细胞浸润,血管增生明显。与模型组比较,凉血活血胶囊组小鼠银屑病样皮损症状缓解,PASI分数降低,表皮角化不全减轻,角质形成增殖减少,T淋巴细胞、树突状细胞、巨噬细胞、中性粒细胞和单核细胞的浸润减弱,新生血管减少。结论:凉血活血胶囊可通过影响表皮细胞过度增殖、角化不全、炎症细胞浸润及血管增生而改善咪喹莫特诱导的小鼠银屑病样皮损变化。

AIM： To observe the effects of Liang Xue Huo Xue（LXHX） capsules on mouse psoriasis-like lesions induced by imiquimod（IMQ）. METHODS： BALB/c female mice（n=48） were randomly divided into 6 groups： normal group,model group,LXHX capsules groups with high,medium or low doses,and glycosides of Tripterygium wilfordii（GTW） group.On day 8,skin lesions were determined by pathological examination.The lesions were evaluated according to the psoriasis area and severity index（PASI）.The histology and epidermal thicknesses were observed under light microscope.The expression of proliferating cell nuclear antigen（PCNA） was detected by immunohistochemical staining.Meanwhile,the positive expression of CD3,CD11c,F4/80,CD31 and Gr-1 was counted by immunohistochemical staining. RESULTS： Compared with model group,the cutaneous symptoms in LXHX capsules groups were alleviated,with PASI scores decreased,epidermal parakeratosis and epidermal over-proliferation relived,the numbers of dermal T lymphocytes,dendritic cells,macrophages,neutrophils and monocytes reduced significantly. CONCLUSION： LXHX capsules improve imiquimod-induced mouse psoriasis-like lesions by inhibiting over-proliferation of keratinocytes,parakeratosis,inflammatory infiltration and angiogenisis.