大鼠自体原位肝移植模型中肠道羟自由基、丙二醛和总抗氧化能力的变化

Changes of hydroxyl radical,malondialdehyde and total antioxidant capa-city in intestines after orthotopic liver autotransplantation in rats

目的:建立SD大鼠自体原位肝移植模型,观察自体模拟肝移植后不同时期肠道黏膜病理学变化及肠组织的羟自由基(.OH)、丙二醛(MDA)和总抗氧化能力(T-AOC)的变化。方法:SD大鼠36只,随机分为假手术组(S组)和模型组(M组),模型组根据肝脏再灌注时间,再分为再灌注2 h模型组(M1组)、再灌注4 h模型组(M2组)、再灌注8 h模型组(M3组)、再灌注16 h模型组(M4组)和再灌注24 h模型组(M5组)5个亚组。对照组在麻醉后只进行开腹和血管的分离,不进行肝脏的阻断和灌注;模型组则进行自体肝移植手术。分别在肝脏再灌注后2、4、8、16、24 h取肠组织,观察其病理形态学变化及.OH、MDA和T-AOC的变化。结果:(1)与S组相比,M组大鼠肠黏膜在肝脏再灌注后出现明显的病理学损伤,可见上皮下间隙的扩大,甚至出现绒毛上皮和固有层分离,再灌注8 h时最为显著,再灌注24 h开始修复;(2)与S组相比,M2、M3和M4组.OH水平明显升高(P<0.05),M1、M2、M3组MDA含量明显升高(P<0.05),M1、M2、M3和M4组T-AOC明显降低(P<0.05)。结论:自体原位肝移植可使大鼠肠黏膜.OH和MDA含量增加,T-AOC下降,肠道出现可逆性病理损伤。

AIM： To explore the pathological changes of small intestines after orthotopic liver autotransplantation in rats and to analyze the correlation between these changes and the levels of hydroxy radical（·OH）,malondialdehyde（MDA）and total antioxidant capacity（T-AOC）.METHODS： Thirty-six Sprague-Dawley rats were randomly divided into sham operation group（group S,n=6） and model group（group M）.According to the period after liver reperfusion,the rats in group M were divided into 5 sub-groups： 2 h after reperfusion（group M1,n=6）,4 h after reperfusion（group M2,n=6）,8 h after reperfusion（group M3,n=6）,16 h after reperfusion（group M4,n=6）,and 24 h after reperfusion（group M5,n=6）.After anesthesia,the rats in group S involved laparotomy and vascular dissection without hepatic vascular exclusion and perfusion.The rats in other groups received orthotopic liver autotransplantation.The intestinal tissues starting from 5 cm to terminal ileum were removed 2 h,4 h,8 h,16 h and 24 h after reperfusion.The morphological changes of intestinal epithelial basement membrane were observed under optical microscope.The levels of ·OH,MDA and T-AOC were detected.RESULTS：（1） In model groups,the morphological damages in the intestines were significant compared to group S,especially 8 h after reperfusion.The intestines showed massive epithelial lifting down the sides of villi and a few tips being denuded.The repair of pathological damage in the intestines 24 h after reperfusion was observed.（2） Compared to group S,the levels of ·OH in the intestines significantly increased in group M2,M3 and M4（P0.05）.The levels of MDA in the intestines significantly increased in group M1,M2 and M3（P0.05）.The levels of T-AOC significantly decreased in group M1,M2,M3 and M4（P0.05）.CONCLUSION： Orthotopic liver autotransplantation increases the levels of ·OH and MDA,diminishes T-AOC and induces reversible pathological changes in intestines.