HPLC法测定江西栀子中绿原酸的含量

Determining content of chlorogenic acid in Jiangxi gardenia by HPLC

目的:测定了江西产栀子中绿原酸的含量。方法:采用外标法。ZorbaxXDB-C18(4.6×150mm,5μm)色谱柱,乙腈—1.1%冰乙酸(0～12min:7∶93;12～20min:10∶90),流速为1.0ml/min,柱温25℃,检测波长为326nm。结果:测得5个产地栀子中绿原酸含量最高为0.114%,最低才0.003%。结论:本研究建立的栀子中绿原酸含量测定方法简便易行,结果准确、可靠,便于对栀子药材进行质量控制。江西不同产地栀子中绿原酸含量由于土壤等各种因素影响,差异明显。

Objective：To develop a method using HPLC for determination content of chlorogenic acid in gardenia from Jiang Xi.Methods：Using the external standard method.Zorbax XDB-C18（4.6×150mm,5μm）,mobile phase：acetonitrile-1.1%acetic acid glacial（10∶90）,detected wavelength：326nm.The column temperature was maintained at 25℃.Results：The highest content of chlorogenic acid of gardenia in 5 origin was 0.114%,and the lowest content was 0.003%.Conclusion：The HPLC method is simply and suitable to control quantitative of gardenia.As the soil and other factors affect chlorogenic acid content in the different origin,the difference is obvious.