幽门螺杆菌cagE和virB11基因与胃癌的关系研究

Relationship between Helicobacter Pylori Genes cagE and virB11 and Gastric Cancer

目的观察幽门螺杆菌毒力相关基因cagE和virB11基因型与胃癌病理分期、淋巴结受累及远端转移的关系。方法应用PCR技术,进行cagE和virB11基因片段的扩增,分析幽门螺杆菌感染的胃癌患者cagE和virB11基因型的检出率。结果胃癌患者中幽门螺杆菌感染率为54.44%(141/259)。cagE基因检出率为57.45%(81/141),virB11基因检出率为65.25%(92/141)。cagE和virB11基因型(+/-、-/+、+/+、-/-)在贲门癌和非贲门癌患者中的检出率明显不同(χ2=14.53,P=0.002),尤其是cagE+/virB11+基因型;与其他基因型相比,cagE+/virB11+基因型在T2、T3期、淋巴结转移(N0、N1)、无远处转移的癌变组织中呈现出高检出率。结论 cagE+/virB11+基因型与胃癌的浸润、早期淋巴结转移、无远处转移密切相关,可能是一种更具致病性的基因型,在胃癌的发生发展过程中起重要作用。

Objective To investigate the frequency of cagE and virB11 genes of H. pylori isolated from patients with gastric cancer and to analyze the association between cagE/virB11 gene subtypes and pathological development. Methods The subtypes of cagE and virBll were detected by PCR from the genomie DNA of 141 patients with gastric carcinoma identified with H. pylori infection. The detection rate of cage and virB11 was analyzed. Results 54.44% （141/259） of the gastric cancer pa- tients were detected with H. pylori. 57.45% （81/141） and 65.25% （92/141） of the tested tumors was positive for cage and virBll respectively. Compared with other gene subtypes, the frequencies of cagE ＋/virBll - , cagE -/virBll ＋ , cage ＋/ virB11 ＋ and cagE -/virB11 - gene subtypes were significantly higher in non -cardia tumors than eardia tumors, especially for cagE ＋/virB11 ＋ gene subtype （X^2 = 14. 53, P = 0. 002） . The frequencies of cagE ＋/virB11 ＋ gene subtype were significantly higher in tumors at the stage of T2 and T3 , lymph node metastasis （ N0 and N1 ） and cancer tissues without distant metasta- sis. Conclusion The subtype of cage ＋/virB11 ＋ is closely related to gastric cancer invasion, early stage lymph node metastasis and non distant metastasis. It might be a gene subtype of high pathogenicity, playing an important role in the occurrence and de- velopment of gastric cancer.