醌氧化还原酶1基因多态性与肝癌易感性关系研究

Association of NQO1 Gene Polymorphism with Susceptibility of Hepatocellular Carcinoma

目的探讨肝癌易感性与醌氧化还原酶1(NQO1)基因多态性的关系。方法以桂林医学院附属医院136例肝癌患者为病例组,选择同期该院的123例无肿瘤患者为对照组,进行肝癌相关危险因素的问卷调查,应用荧光探针(Taqman MGB)技术检测NQO1基因609位点的多态性。结果 NQO1基因3种基因型在两组间差异有统计学意义(χ2=17.345,P<0.05),且携带有NQO1突变杂合子(C/T)和突变纯合子(T/T)的个体发生肝癌的危险性较携带野生纯合子(C/C)个体高,NQO1基因携带突变基因型T与经常吸烟可以增加罹患肝癌风险[OR=2.643,95%CI(1.379,5.066)]。结论 NQO1基因多态性在肝癌发生过程中,突变基因型可能是一个危险因素,且与吸烟存在协同作用。

Objective To study the relationship between the polymorphisms of NQO1 and the susceptibility of hepatocellular carcinoma(HCC). Methods The research was a case-control study which included 136 cases of HCC in case group and 123 cases without tumor in control group,all receiving questionnaires about the risk factors for liver cancer.609 sites of NQO1 polymorphisms were determined by polymerase chain restriction with Taqman MGB probe. Results Three types of gene among NQO1 were significantly different between the two groups(χ2=17.345,P<0.05)and the cases with mutation homozygote(C/T)and mutation heterozygote(T/T)had higher risks for liver cancer than those wild homozygote.NQ01 with mutation allele(T)and smoking were high risk factors for liver cancer[OR=2.643,95%CI(1.379,5.066)]. Conclusion NQO1 mutation genotype may be a risk factor and will have a synergistic influence together with smoking in the formation of liver cancer.