半夏泻心汤对反流性食管炎大鼠食道黏膜免疫机能影响的研究

Research of Pinelliae Decoction for Purging Stomach-Fire on Immune Function of Esophagus Mucous Membrane in Rat Model of Reflux Esophagitis

目的:探讨半夏泻心汤对实验性反流性食管炎大鼠模型食道黏膜结构、细胞间黏附分子-1、L-选择素的影响。方法:将160只大鼠随机分为假手术组、模型组、中药组和西药组,每组40只,应用光镜观察各组食道黏膜的病理分级情况,应用免疫组化方法检测细胞间黏附分子-1和L-选择素的表达情况。结果:中药组和西药组食道黏膜病理分级明显优于模型组(u=7.135 6、7.271 9,P=0.000 0),中药组和西药组之间差异无统计学意义;免疫组织化学显示模型组大鼠食管黏膜下层及肌层间质中小血管的内皮细胞上细胞间黏附分子和L-选择素呈较强表达,中药组和西药组仅呈弱表达,假手术组呈阴性表达。结论:半夏泻心汤可缓解反流性食管炎大鼠模型食道黏膜损伤、降低细胞间黏附分子-1和L-选择素的表达。

Objective：To investigate the effect of Pinelliae Decoction for Purging Stomach-Fire on mucous membrane structure of esophagus, intercellular adhesion-molecule-1 among cells and L-selectin in the rat model of experimental reflux esophagitis. Methods ：160 rats were divided into four groups,which were sham operated group, model control group, Chinese medical group and western medical group. There were 40 rats in each group. Pathological grading of the esophagus mucous membrane in each group was observed with light microscope. And the expression of intercellular adhesion-molecule-1 and L-selectin were detected with immunohistochemistry. Results：The pathological grading of the esophagus mucous membrane in Chinese medical group and western medical group were better than that in model control group （u= 7.135 6,7.271 9, P = 0. 000 0）, but there was not significant differ- ence between Chinese medical group and western medical group. Immunohistochemistry showed that the expressions of intercellular adhesion molecule-1 and L-selectin in interstitial vascular endothelial cells of esophageal submucosa and muscle layer were strong in model control group, but weak in Chinese medical group and western medical group, and negative in sham operated group. Conclusion：Pinelliae Decoction for Purging Stomach-Fire can help relieve esophageal mucosa injury, and reduces the expression of intercellular adhesion molecule-1 and L-selectin.