rhIL-1α与1,25-(OH)_2D_3联合作用对人牙周膜成纤维细胞表达RANKL和OPG的影响

Effects of Recombinant Human Interleukin-1α and 1,25-(OH)2D3 on the Gene Expression of RANKL and OPG in HPDLFs

目的:研究rhIL-1α与1,25-(OH)2D3联合作用对HPDLFs表达RANKL和OPG的影响,探讨牙槽骨改建的调节机制。方法:10μg/L rhIL-1α与1×10-8 mol/L1,25-(OH)2D3联合作用于体外培养的HPDLFs,于48h后收集细胞,利用荧光定量RT-PCR技术检测RANKL mRNA和OPG mRNA的表达,探讨RANKL/OPG比值的变化。结果:rhIL-1α和1,25-(OH)2D3都调节HPDLFs表达RANKL和OPG。rhIL-1α单独作用上调HPDLFs表达RANKL和OPG,增加RANKL/OPG比值(P<0.05);1,25-(OH)2D3单独作用则上调表达RANKL,下调表达OPG,增加RANKL/OPG比值(P<0.05)。这2种调节因子联合作用对HPDLFs RANKL表达有协同作用,但对RANKL/OPG比值的影响无协同效应。结论:rhIL-1α和1,25-(OH)2D3都通过RANKL-OPG途径调节HPDLFs参与牙槽骨改建,2种调节因子联合作用对RANKL表达的影响明显优于单因素诱导效果,但对RANKL/OPG比值的影响并没有产生协同效应或累加效应。

Objective： To evaluate the effects of rhIL-1α and 1,25-（OH）2D3 on the expression of RANKL and OPG in HPDLFs and to discuss the change of ratio of RANKL/OPG.Methods： HPDLFs were treated with 10ng/ml rhIL-1α,1×10-8mol/L 1,25-（OH）2D3 or 10ng/ml rhIL-1α plus 1×10-8mol/L 1,25-（OH）2D3 for 48 hours.The expression of RANKL and OPG were measured by FQ-RT-PCR.Results： RhIL-1α and 1,25（OH）2D3 influenced the expression of RANKL and OPG in HPDLFs.The expression of RANKL and OPG were increased with rhIL-1α（P〈0.05）.Moreover,1,25（OH）2D3 up regulated the expression of RANKL and decreased the expression of OPG,so the ratio of RANKL/OPG was increased too（P〈0.05）.There was cumulative effect on regulating the expression of RANKLwith the synergistic use of rhIL-1αand 1,25（OH）2D3,but there was no cumulative effect on the ratio of RANKL/OPG.Conclusion： RhIL-1α and 1,25-（OH）2D3 act through RANKL-OPG pathway to regulate the alveolar bone remolding.There was additive effect on the expression of RANKL with combination use of rhIL-1α and 1,25-（OH）2D3,but there was no additive effect or synergistic effect on the ratio of RANKL/OPG.