X射线致小鼠急性损伤的观察

Observation of acute radiation damage on mice exposed to X-rays

本研究用6 Gy X射线对小鼠进行全身一次性照射,分别在照射后1、3和7 d经颈椎脱臼处死,测定小鼠外周血红、白细胞数量,胸腺指数和脾脏指数,骨髓嗜多染红细胞微核形成率以及肝脏抗氧化水平。结果表明:照射24 h后,小鼠外周血白细胞数量急剧降低,胸腺指数和脾脏指数下降,骨髓嗜多染红细胞微核形成率增加,肝脏脂质过氧化水平上升。放射7 d后,胸腺指数稍有回升,骨髓红细胞微核形成率和肝脏脂质过氧化水平略为降低,其它指标随时间变化损伤程度持续增加。

Mice were whole-body exposed to X-rays with absorbed dose of 6 Gy and sacrificed on the 1 st, 3rd and 7th day by cervical dislocation after irradiation, respectively. The number of red blood cells and white blood cells in peripheral blood, the index of thymus and spleen, the micronucleus rate of bone marrow polychromatic erythro- blasts （PCE） and the level of antioxidant of liver were determined. It was observed that white blood cells decreased, the index of thymus and spleen also decreased meanwhile both of the micronucleus rate of bone marrow PCE and level of lipid peroxidation of liver increased at 24 h after irradiation. However, the index of thymus was up slightly on the 7th day after irradiation, while the PCE and the level of lipid peroxidation of liver got down slightly. The other index of injured mice increased continually with time.